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Literature summary for 3.4.23.45 extracted from

  • Stockley, J.H.; Ravid, R.; ONeill, C.
    Altered beta-secretase enzyme kinetics and levels of both BACE1 and BACE2 in the Alzheimers disease brain (2006), FEBS Lett., 580, 6550-6560.
    View publication on PubMed
Search for more literature for 3.4.23.45

Cloned(Commentary)

Cloned (Comment) Organism
expressed as C-terminal Myc-His fusion protein in HEK 293 cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Lys-Thr-Glu-Glu-Ile-Ser-Glu-Val-Asn-Stat-Val-Ala-Glu-Phe P10-P40 StatVal, beta-secretase inhibitor Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 x * 60000, SDS-PAGE, enzyme expressed in HEK 293 Homo sapiens
73000
-
 x * 73000, SDS-PAGE, brain sample Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
amyloid precursor protein + H2O Rattus norvegicus
-
 fragments of amyloid precursor protein
-
 ?
amyloid precursor protein + H2O Homo sapiens can cleave the amyloid precursor protein (APP) at the N terminal Asp-1 position of A-beta, preferential cleavage within the A-beta region at Phe19 or Phe20, enzyme function is not consistent with a requirement and function of BACE1 as a beta-secretase in the brain, may act antagonistically to BACE1 fragments of amyloid precursor protein soluble ectodomain (sAPPb),and a membrane-bound APP C-terminal fragment of 99 amino acids ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 patients with Alzheimer’s disease, BACE1 and BACE2 levels are found to be decreased in Alzheimer’s disease patients
-
Rattus norvegicus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
broad endopeptidase specificity. Cleaves Glu-Val-Asn-Leu-/-Asp-Ala-Glu-Phe in the Swedish variant of Alzheimer's amyloid precursor protein preferential cleavage within the A-beta region at Phe19 or Phe20 Homo sapiens
a

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
-
brain mid-temporal cortex, low mRNA level compared with BACE1 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(MCA)Glu-Val-Lys-Met-Asp-Ala-Glu-Phe-Lys(DNP) + H2O synthetic peptide substrate based on the beta-secretase cleavage site of wild-type APP Homo sapiens (MCA)Glu-Val-Lys-Met + Asp-Ala-Glu-Phe-Lys(DNP)
-
 ?
amyloid precursor protein + H2O
-
 Rattus norvegicus fragments of amyloid precursor protein
-
 ?
amyloid precursor protein + H2O can cleave the amyloid precursor protein (APP) at the N terminal Asp-1 position of A-beta, preferential cleavage within the A-beta region at Phe19 or Phe20, enzyme function is not consistent with a requirement and function of BACE1 as a beta-secretase in the brain, may act antagonistically to BACE1 Homo sapiens fragments of amyloid precursor protein soluble ectodomain (sAPPb),and a membrane-bound APP C-terminal fragment of 99 amino acids ?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE, enzyme expressed in HEK 293 Homo sapiens
? x * 73000, SDS-PAGE, brain sample Rattus norvegicus
? x * 73000-75000, SDS-PAGE, brain sample Homo sapiens

Synonyms

Synonyms Comment Organism
BACE2
-
 Rattus norvegicus
BACE2 homologue to BACE1 Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000041
-
 pH 4.5, 37°C, substrate concentration 0.015 mM Homo sapiens Lys-Thr-Glu-Glu-Ile-Ser-Glu-Val-Asn-Stat-Val-Ala-Glu-Phe