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Literature summary for 3.4.23.43 extracted from

  • Aly, K.A.; Beebe, E.T.; Chan, C.H.; Goren, M.A.; Sepulveda, C.; Makino, S.; Fox, B.G.; Forest, K.T.
    Cell-free production of integral membrane aspartic acid proteases reveals zinc-dependent methyltransferase activity of the Pseudomonas aeruginosa prepilin peptidase PilD (2013), MicrobiologyOpen, 2, 94-104.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis scaled-up synthesis of PilD, followed by solubilization in dodecyl-beta-D-maltoside and chromatography, leads to a pure enzyme that retains its known biochemical activities Pseudomonas aeruginosa

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ PilD is a zinc-binding protein. Zinc is required for the N-terminal methylation of the mature pilin, but not for signal peptide cleavage Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas aeruginosa enzyme additionally catalyzes S-adenosyl methionine-dependent methylation of the mature pilin ?
-
?
additional information Pseudomonas aeruginosa ATCC 15692 enzyme additionally catalyzes S-adenosyl methionine-dependent methylation of the mature pilin ?
-
?
prepilin PilA + H2O Pseudomonas aeruginosa
-
? cosynthesis of PilD with its full-length substrate, PilA, leads to complete cleavage of the substrate signal peptides ?
prepilin PilA + H2O Pseudomonas aeruginosa ATCC 15692
-
? cosynthesis of PilD with its full-length substrate, PilA, leads to complete cleavage of the substrate signal peptides ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa P22610
-
-
Pseudomonas aeruginosa ATCC 15692 P22610
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme additionally catalyzes S-adenosyl methionine-dependent methylation of the mature pilin Pseudomonas aeruginosa ?
-
?
additional information enzyme additionally catalyzes S-adenosyl methionine-dependent methylation of the mature pilin Pseudomonas aeruginosa ATCC 15692 ?
-
?
prepilin PilA + H2O
-
Pseudomonas aeruginosa ? cosynthesis of PilD with its full-length substrate, PilA, leads to complete cleavage of the substrate signal peptides ?
prepilin PilA + H2O
-
Pseudomonas aeruginosa ATCC 15692 ? cosynthesis of PilD with its full-length substrate, PilA, leads to complete cleavage of the substrate signal peptides ?

Synonyms

Synonyms Comment Organism
PilD
-
Pseudomonas aeruginosa