Literature summary for 3.4.23.4 extracted from
Eskandari, M.; Hosseini, A.; Alasvand Zarasvand, S.; Aminlari, M.
Cloning, expression, purification and refolding of caprine prochymosin (2012), Food Biotechnol., 26, 143-153.
No PubMed abstract available
Application
Application |
Comment |
Organism |
food industry |
the enzyme is used as milk coagulant in the cheese industry |
Capra hircus |
Cloned(Commentary)
Cloned (Comment) |
Organism |
expressed in Escherichia coli BL21(DE3) cells |
Capra hircus |
General Stability
General Stability |
Organism |
for prochymosins activation, the supernatant is adjusted to pH 2.0 and incubated for 2 h at 25°C, then adjusted to pH 6.3 and left again at 25°C for 1 h |
Capra hircus |
Molecular Weight [Da]
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
42000 |
- |
x * 42000, recombinant enzyme, SDS-PAGE |
Capra hircus |
Natural Substrates/ Products (Substrates)
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
kappa-casein + H2O |
Capra hircus |
cleavage between Phe105-Met106 |
para-kappa-casein + glycopeptide |
- |
? |
|
Organism
Organism |
UniProt |
Comment |
Textmining |
Capra hircus |
Q5VK60 |
- |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
metal affinity chromatography |
Capra hircus |
Renatured (Commentary)
Renatured (Comment) |
Organism |
using 8 M urea, at 25°C |
Capra hircus |
Source Tissue
Source Tissue |
Comment |
Organism |
Textmining |
abomasum |
- |
Capra hircus |
- |
stomach |
- |
Capra hircus |
- |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
kappa-casein + H2O |
cleavage between Phe105-Met106 |
Capra hircus |
para-kappa-casein + glycopeptide |
- |
? |
|
Subunits
Subunits |
Comment |
Organism |
? |
x * 42000, recombinant enzyme, SDS-PAGE |
Capra hircus |
Synonyms
Synonyms |
Comment |
Organism |
prochymosin |
- |
Capra hircus |
rennin |
- |
Capra hircus |