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Literature summary for 3.4.23.4 extracted from

  • Eskandari, M.; Hosseini, A.; Alasvand Zarasvand, S.; Aminlari, M.
    Cloning, expression, purification and refolding of caprine prochymosin (2012), Food Biotechnol., 26, 143-153.
No PubMed abstract available

Application

Application Comment Organism
food industry the enzyme is used as milk coagulant in the cheese industry Capra hircus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Capra hircus

General Stability

General Stability Organism
for prochymosins activation, the supernatant is adjusted to pH 2.0 and incubated for 2 h at 25°C, then adjusted to pH 6.3 and left again at 25°C for 1 h Capra hircus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
x * 42000, recombinant enzyme, SDS-PAGE Capra hircus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
kappa-casein + H2O Capra hircus cleavage between Phe105-Met106 para-kappa-casein + glycopeptide
-
?

Organism

Organism UniProt Comment Textmining
Capra hircus Q5VK60
-
-

Purification (Commentary)

Purification (Comment) Organism
metal affinity chromatography Capra hircus

Renatured (Commentary)

Renatured (Comment) Organism
using 8 M urea, at 25°C Capra hircus

Source Tissue

Source Tissue Comment Organism Textmining
abomasum
-
Capra hircus
-
stomach
-
Capra hircus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
kappa-casein + H2O cleavage between Phe105-Met106 Capra hircus para-kappa-casein + glycopeptide
-
?

Subunits

Subunits Comment Organism
? x * 42000, recombinant enzyme, SDS-PAGE Capra hircus

Synonyms

Synonyms Comment Organism
prochymosin
-
Capra hircus
rennin
-
Capra hircus