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Literature summary for 3.4.23.4 extracted from

  • Justesen, S.F.; Lamberth, K.; Nielsen, L.L.; Schafer-Nielsen, C.; Buus, S.
    Recombinant chymosin used for exact and complete removal of a prochymosin derived fusion tag releasing intact native target protein (2009), Protein Sci., 18, 1023-1032.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis use of chymosin to cleave a pro-chymosin derived fusion tag releasing native target proteins. After modification of the pro-chymosin fusion tag chymosin can remove this tag at more neutral pH 6.2, less prone to compromise the integrity of target proteins. Chymosin produces intact native target protein both at the level of small and large-scale preparations Bos taurus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Aspergillus niger Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
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-
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
YGISSKFCE + H2O modified peptide based on prochymosin sequence Bos taurus YGISSKF + L-Cys-L-Glu 100% cleavage at enzyme to substrate ratio of 1:1, pH 6.2, 16 h ?
YGISSKFfE + H2O modified peptide based on prochymosin sequence Bos taurus YGISSKF + FE 100% cleavage at enzyme to substrate ratio of 1:1, pH 6.2, 16 h ?
YGISSKFHE + H2O modified peptide based on prochymosin sequence Bos taurus YGISSKF + His-Glu 51% cleavage at enzyme to substrate ratio of 1:1, pH 6.2, 16 h ?
YGISSKFIE + H2O modified peptide based on prochymosin sequence Bos taurus YGISSKF + Ile-Glu 54% cleavage at enzyme to substrate ratio of 1:1, pH 6.2, 16 h ?
YGISSKFKE + H2O modified peptide based on prochymosin sequence Bos taurus YGISSKF + Lys-Glu 33% cleavage at enzyme to substrate ratio of 1:1, pH 6.2, 16 h ?
YGISSKFLE + H2O modified peptide based on prochymosin sequence Bos taurus YGISSKF + L-Leu-L-Glu 42% cleavage at enzyme to substrate ratio of 1:1, pH 6.2, 16 h ?
YGISSKFME + H2O modified peptide based on prochymosin sequence Bos taurus YGISSKF + L-Met-L-Glu 52% cleavage at enzyme to substrate ratio of 1:1, pH 6.2, 16 h ?
YGISSKFNE + H2O modified peptide based on prochymosin sequence Bos taurus YGISSKF + L-Asn-L-Glu 33% cleavage at enzyme to substrate ratio of 1:1, pH 6.2, 16 h ?
YGISSKFRE + H2O modified peptide based on prochymosin sequence Bos taurus YGISSKF + Arg-Glu 59% cleavage at enzyme to substrate ratio of 1:1, pH 6.2, 16 h ?
YGISSKFVE + H2O modified peptide based on prochymosin sequence Bos taurus YGISSKF + Val-Glu 36% cleavage at enzyme to substrate ratio of 1:1, pH 6.2, 16 h ?
YGISSKFWE + H2O modified peptide based on prochymosin sequence Bos taurus YGISSKF + Trp-Glu 100% cleavage at enzyme to substrate ratio of 1:1, pH 6.2, 16 h ?
YGISSKFYE + H2O modified peptide based on prochymosin sequence Bos taurus YGISSKF + L-Tyr-L-Glu 100% cleavage at enzyme to substrate ratio of 1:1, pH 6.2, 16 h ?