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Literature summary for 3.4.23.4 extracted from

  • Kappeler, S.R.; van den Brink, H.J.; Rahbek-Nielsen, H.; Farah, Z.; Puhan, Z.; Hansen, E.B.; Johansen, E.
    Characterization of recombinant camel chymosin reveals superior properties for the coagulation of bovine and camel milk (2006), Biochem. Biophys. Res. Commun., 342, 647-654.
    View publication on PubMed
Search for more literature for 3.4.23.4

Application

Application Comment Organism
additional information under typical cheese-making conditions (pH 6.6, 0-2 mM CaCl2) the clotting activity of camel chymosin is ca. 80% higher than the activity of bovine chymosin (average clotting activity of camel chymosin is 70% higher than the activity of bovine chymosin), camel chymosin is more thermostable than bovine chymosin Camelus dromedarius

Cloned(Commentary)

Cloned (Comment) Organism
expression in Aspergillus niger var. awamori dgr246pyrG Camelus dromedarius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.056
-
 undecapeptide analogue to chymosin sensitive region of camel kappa-casein
-
 Camelus dromedarius
0.077
-
 undecapeptide analogue to chymosin sensitive region of bovine kappa-casein
-
 Camelus dromedarius
0.134
-
 undecapeptide analogue to chymosin sensitive region of camel kappa casein
-
 Bos taurus
0.165
-
 undecapeptide analogue to chymosin sensitive region of bovine kappa casein
-
 Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35600
-
 calculated peptide mass, SDS-PAGE Camelus dromedarius
40000
-
 apparent molecular weight, determined by comparison to a Novex Mark12TM Camelus dromedarius

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Camelus dromedarius
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by affinity chromatography MIMO1300 matrix Camelus dromedarius

Source Tissue

Source Tissue Comment Organism Textmining
abomasum
-
 Camelus dromedarius
-
epithelial cell
-
 Camelus dromedarius
-
mucosa
-
 Camelus dromedarius
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alphaS-casein + H2O
-
 Bos taurus ?
-
 ?
alphaS-casein + H2O
-
 Camelus dromedarius ?
-
 ?
beta-casein + H2O
-
 Bos taurus ?
-
 ?
beta-casein + H2O
-
 Camelus dromedarius ?
-
 ?
undecapeptide analogue to chymosin sensitive region of bovine kappa casein synthetic substrate Bos taurus ?
-
 ?
undecapeptide analogue to chymosin sensitive region of bovine kappa-casein synthetic substrate Camelus dromedarius ?
-
 ?
undecapeptide analogue to chymosin sensitive region of bovine kappa-casein + H2O
-
 Camelus dromedarius ?
-
 ?
undecapeptide analogue to chymosin sensitive region of camel kappa casein synthetic substrate Bos taurus ?
-
 ?
undecapeptide analogue to chymosin sensitive region of camel kappa-casein synthetic substrate Camelus dromedarius ?
-
 ?
undecapeptide analogue to chymosin sensitive region of camel kappa-casein + H2O
-
 Camelus dromedarius ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
42
-
 for proteolysis of the undecapeptide analogue to chymosin sensitive region of bovine kappa casein Bos taurus
42
-
 for proteolysis of the undecapeptide analogue to chymosin sensitive region of bovine kappa casein Camelus dromedarius
47
-
 for proteolysis of the undecapeptide analogue to chymosin sensitive region of camel kappa casein Camelus dromedarius
56
-
 for proteolysis of the undecapeptide analogue to chymosin sensitive region of camel kappa casein Bos taurus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5 55
-
 Camelus dromedarius
5 52.5
-
 Bos taurus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 55 no residual clotting activities at temperatures higher than 55°C Camelus dromedarius

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.3
-
 undecapeptide analogue to chymosin sensitive region of camel kappa casein
-
 Bos taurus
5.1
-
 undecapeptide analogue to chymosin sensitive region of camel kappa-casein
-
 Camelus dromedarius
11.7
-
 undecapeptide analogue to chymosin sensitive region of bovine kappa-casein
-
 Camelus dromedarius
44.3
-
 undecapeptide analogue to chymosin sensitive region of bovine kappa casein
-
 Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.9
-
 for proteolysis of undecapeptide analogue to chymosin sensitive region of the bovine kappa casein Bos taurus
5.1
-
 for proteolysis of the undecapeptide analogue to chymosin sensitive region of bovine kappa casein Camelus dromedarius

pH Range

pH Minimum pH Maximum Comment Organism
2 6.7
-
 Bos taurus
2 6.7
-
 Camelus dromedarius
4.85
-
 calculated isoelectric point Camelus dromedarius
5.5
-
 measured isoelectric point Camelus dromedarius

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 3.8
-
 Camelus dromedarius