Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Plasmodium falciparum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the role of the prosegment in aspartic proteinase structure and function is investigated by generating a chimeric protein consisting of the porcine pepsinogen prosegment fused to the mature region of PMII (pepproPMII): Chimeric protein pepproPMII is capable of both autoactivation and hydrolysis of a synthetic substrate. CD measurements indicate that pepproPMII has reduced thermal stability when compared with zymogen PMII. Prosegment is not critical to obtain a functional enzyme but likely plays a role in protein stabilization | Plasmodium falciparum |
additional information | the role of the prosegment in aspartic proteinase structure and function is investigated by generating a chimeric protein consisting of the prosegment of PMII fused to pepsin (PMIIpropep). Chimeric protein is expressed in Escherichia coli but extremely unstable suggesting protein misfolding | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
pepstatin A | inhibits autoactivation | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00017 | - |
EDANS-CO-CH2-CH2-CO-Ala-Leu-Glu-Arg-Met-Phe-Leu-Ser-Phe-Pro-Dap-(DABCYL)-OH | PMII without prosegment, pH 5, temperature not specified in the publication | Plasmodium falciparum | |
0.0007 | - |
EDANS-CO-CH2-CH2-CO-Ala-Leu-Glu-Arg-Met-Phe-Leu-Ser-Phe-Pro-Dap-(DABCYL)-OH | chimeric PMII (porcine pepsinogen prosegment fused to the mature region of PMII), pH 5, temperature not specified in the publication | Plasmodium falciparum | |
0.0012 | - |
EDANS-CO-CH2-CH2-CO-Ala-Leu-Glu-Arg-Met-Phe-Leu-Ser-Phe-Pro-Dap-(DABCYL)-OH | wild-type PMII, pH 5, temperature not specified in the publication | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | protein is expressed as an inactive zymogen with a large prosegment | Plasmodium falciparum |
Purification (Comment) | Organism |
---|---|
using nickel affinity chromatography and gel filtration | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
EDANS-CO-CH2-CH2-CO-Ala-Leu-Glu-Arg-Met-Phe-Leu-Ser-Phe-Pro-Dap-(DABCYL)-OH + H2O | quenched fluorescent peptide substrate | Plasmodium falciparum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
plasmepsin II | - |
Plasmodium falciparum |
PMII | - |
Plasmodium falciparum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.103 | - |
EDANS-CO-CH2-CH2-CO-Ala-Leu-Glu-Arg-Met-Phe-Leu-Ser-Phe-Pro-Dap-(DABCYL)-OH | PMII without prosegment, pH 5, temperature not specified in the publication | Plasmodium falciparum | |
1.11 | - |
EDANS-CO-CH2-CH2-CO-Ala-Leu-Glu-Arg-Met-Phe-Leu-Ser-Phe-Pro-Dap-(DABCYL)-OH | chimeric PMII (porcine pepsinogen prosegment fused to the mature region of PMII), pH 5, temperature not specified in the publication | Plasmodium falciparum | |
2.1 | - |
EDANS-CO-CH2-CH2-CO-Ala-Leu-Glu-Arg-Met-Phe-Leu-Ser-Phe-Pro-Dap-(DABCYL)-OH | wild-type PMII, pH 5, temperature not specified in the publication | Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
optimal pH of wild-type PMII, chimeric PMII and PMII without prosegment | Plasmodium falciparum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 5 | the zpepproPMII chimeric protein (porcine pepsinogen prosegment fused to the mature region of PMII) activates at pH 3-5. Activation at these pH values is consistent with the pH-dependent autoactivation observed for wildtype zymogen PMII | Plasmodium falciparum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
620 | - |
EDANS-CO-CH2-CH2-CO-Ala-Leu-Glu-Arg-Met-Phe-Leu-Ser-Phe-Pro-Dap-(DABCYL)-OH | PMII without prosegment, pH 5, temperature not specified in the publication | Plasmodium falciparum | |
1500 | - |
EDANS-CO-CH2-CH2-CO-Ala-Leu-Glu-Arg-Met-Phe-Leu-Ser-Phe-Pro-Dap-(DABCYL)-OH | wild-type PMII, pH 5, temperature not specified in the publication | Plasmodium falciparum | |
1660 | - |
EDANS-CO-CH2-CH2-CO-Ala-Leu-Glu-Arg-Met-Phe-Leu-Ser-Phe-Pro-Dap-(DABCYL)-OH | chimeric PMII (porcine pepsinogen prosegment fused to the mature region of PMII), pH 5, temperature not specified in the publication | Plasmodium falciparum |