Application | Comment | Organism |
---|---|---|
molecular biology | plasmepsin 2 but not plasmepsin 4 is a pential tool for hydrogen/deuterium exchange coupled to mass spectroscopy (DXMS) studies | Plasmodium falciparum |
Cloned (Comment) | Organism |
---|---|
PSM2 (residues 78-453) are recombinantly expressed | Plasmodium falciparum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
activated form | Plasmodium falciparum |
43000 | - |
precursor form | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
plasmepsin 2 + H2O | Plasmodium falciparum | PSM2 auto-cleavage after F112 is complete after 20 min in 10 mM citrate pH 4.5 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | PSM2 is less active after maturation. At pH 2.2 PSM2 activity is observed only without maturation | Plasmodium falciparum |
Purification (Comment) | Organism |
---|---|
- |
Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Hemoglobin + H2O | bovine and human hemoglobin are used | Plasmodium falciparum | ? | - |
? | |
Myoglobin + H2O | horse myoglobin is used | Plasmodium falciparum | ? | - |
? | |
p47phox-PX + H2O | PX domain of p47phox, a cytosolic factor of the neutrophil NADPH oxydase is digested | Plasmodium falciparum | ? | - |
? | |
plasmepsin 2 + H2O | PSM2 auto-cleavage after F112 is complete after 20 min in 10 mM citrate pH 4.5 | Plasmodium falciparum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
plasmepsin 2 | - |
Plasmodium falciparum |
PSM2 | - |
Plasmodium falciparum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
assay at room temperature. At pH 4.5 the number of cleavage sites is lower at 0°C than at room temperature | Plasmodium falciparum |
- |
- |
assay at, at pH 4.5 the number of cleavage sites is lower at 0°C than at room temperature | Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2.2 | - |
assay at, PSM2 produces more cleavage sites at pH 4.5 than at pH 2.2. At pH 2.2 PSM2 activity is observed only without maturation | Plasmodium falciparum |
4.5 | - |
assay at, at pH 4.5 the number of cleavage sites is lower at 0°C than at room temperature. PSM2 produces more cleavage sites at pH 4.5 than at pH 2.2 | Plasmodium falciparum |