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Literature summary for 3.4.23.39 extracted from

  • Hill, J.; Tyas, L.; Phylip, L.H.; Kay, J.; Dunn, B.M.; Berry, C.
    High level expression and characterisation of plasmepsin II, an aspartic proteinase from Plasmodium falciparum (1994), FEBS Lett., 352, 155-158.
    View publication on PubMed
Search for more literature for 3.4.23.39

Cloned(Commentary)

Cloned (Comment) Organism
DNA encoding the last 48 residues of the propart and the whole mature sequence of plasmepsin II is inserted into the T7 dependent vector pET 3a for high level expression in Escherichia coli Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
Ac-Val-Val-Sta-Ala-Sta IC50: 0.6 nM Plasmodium falciparum
butyloxycarbonyl-His-Pro-Phe-His-Leu-PSI[CH(OH)-CH2]-Val-Ile-His IC50: 150 nM Plasmodium falciparum
butyloxycarbonyl-His-Pro-Phe-His-Sta-Leu-Phe-NH2 IC50: 1000 nM Plasmodium falciparum
Iva-Val-Val-Sta-Ala-Sta IC50: 0.1 nM Plasmodium falciparum
Lac-Val-Sta-Ala-Sta IC50: 100 mM Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 Lys-Pro-Ile-Val-Phe-Phe(NO2)-Arg-Leu pH 4.7 Plasmodium falciparum
0.11
-
 Ala-Leu-Glu-Arg-Thr-Phe-Phe(NO2)-Ser-Phe-Pro-Thr pH 4.7 Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Leu-Glu-Arg-Thr-Phe-Leu-Ser-Phe-Pro-Thr + H2O
-
 Plasmodium falciparum Ala-Leu-Glu-Arg-Thr-Phe + Leu-Ser-Phe-Pro-Thr
-
 ?
Ala-Leu-Glu-Arg-Thr-Phe-Phe(NO2)-Ser-Phe-Pro-Thr + H2O
-
 Plasmodium falciparum Ala-Leu-Glu-Arg-Thr-Phe + Phe(NO2)-Ser-Phe-Pro-Thr
-
 ?
Lys-Pro-Ile-Lys-Phe-Phe(NO2)-Arg-Leu + H2O
-
 Plasmodium falciparum Lys-Pro-Ile-Lys-Phe + Phe(NO2)-Arg-Leu
-
 ?
Lys-Pro-Ile-Val-Phe-Phe(NO2)-Arg-Leu + H2O
-
 Plasmodium falciparum Lys-Pro-Ile-Val-Phe + Phe(NO2)-Arg-Leu
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.04
-
 Lys-Pro-Ile-Lys-Phe-Phe(NO2)-Arg-Leu pH 4.7, 37°C Plasmodium falciparum
1.6
-
 Lys-Pro-Ile-Val-Phe-Phe(NO2)-Arg-Leu pH 4.7 Plasmodium falciparum
3.8
-
 Ala-Leu-Glu-Arg-Thr-Phe-Phe(NO2)-Ser-Phe-Pro-Thr pH 4.7 Plasmodium falciparum

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0000001
-
 IC50: 0.1 nM Plasmodium falciparum Iva-Val-Val-Sta-Ala-Sta
0.0000006
-
 IC50: 0.6 nM Plasmodium falciparum Ac-Val-Val-Sta-Ala-Sta
0.00015
-
 IC50: 150 nM Plasmodium falciparum butyloxycarbonyl-His-Pro-Phe-His-Leu-PSI[CH(OH)-CH2]-Val-Ile-His
0.001
-
 IC50: 1000 nM Plasmodium falciparum butyloxycarbonyl-His-Pro-Phe-His-Sta-Leu-Phe-NH2
100
-
 IC50: 100 mM Plasmodium falciparum Lac-Val-Sta-Ala-Sta