Cloned (Comment) | Organism |
---|---|
expression of proplasmepsin I and V110P-proplasmepsin in Escherichia coli | Plasmodium falciparum |
initial attempts to express full-length proplasmepsin I gene in Escherichia coli results in very low expression levels, possibly due to the toxic effects of the hydrophobic segment, and no activation to mature plasmepsin I is ever observed. Recombinant proplasmepsin I is expressed from pETPMI, a construct that is prepared from pET3a and contains the last 48 residues of the propart followed by the 328 residues of mature plasmepsin I. It is necessary to introduce a processing site, V110P, into the zymogen that renders the precursor capable of undergoing autoactivation | Plasmodium falciparum |
Protein Variants | Comment | Organism |
---|---|---|
V110P | mutation in proplasmepsin I is responsible for generating a form of proplasmepsin I that is capable of undergoing autoactivation | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
isovaleryl-pepstatin | - |
Plasmodium falciparum | |
N'1-[(E)-(2,3-dihydroxyphenyl)methylidene]-N'3-[(Z)-(2,3-dihydroxyphenyl)methylidene]benzene-1,3-dicarbohydrazide | - |
Plasmodium falciparum | |
Ro40-4388 | - |
Plasmodium falciparum | |
Ro40-5576 | - |
Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
Leu-Glu-Arg-Ile-Phe-Phe(NO2)-Ser-Phe | pH 4.4, 37°C | Plasmodium falciparum | |
0.03 | - |
Leu-Glu-Arg-Val-Phe-Phe(NO2)-Ser-Phe | pH 4.4, 37°C | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | plasmepsin I is produced as a precursor. The propart region, about 120 residues, is more than twice as long as those of archetypal zymogens | Plasmodium falciparum |
Purification (Comment) | Organism |
---|---|
recombinant V110P-proplasmepsin I | Plasmodium falciparum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | proplasmepsin I is expressed in the ring stages whereas the proplasmepsin II gene is not transcribed until the later trophozoite stage of parasite growth | Plasmodium falciparum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Leu-Glu-Arg-Thr-Phe-Leu-Ser-Phe-Pro-Thr + H2O | - |
Plasmodium falciparum | Ala-Leu-Glu-Arg-Thr-Phe + Leu-Ser-Phe-Pro-Thr | - |
? | |
Leu-Glu-Arg-Ile-Phe-Phe(NO2)-Ser-Phe + H2O | - |
Plasmodium falciparum | Leu-Glu-Arg-Ile-Phe + Phe(NO2)-Ser-Phe | - |
? | |
Leu-Glu-Arg-Val-Phe-Phe(NO2)-Ser-Phe + H2O | - |
Plasmodium falciparum | Leu-Glu-Arg-Val-Phe + Phe(NO2)-Ser-Phe | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
Leu-Glu-Arg-Ile-Phe-Phe(NO2)-Ser-Phe | pH 4.4, 37°C | Plasmodium falciparum | |
1.6 | - |
Leu-Glu-Arg-Val-Phe-Phe(NO2)-Ser-Phe | pH 4.4, 37°C | Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
hydrolysis of Leu-Glu-Arg-Ile-Phe-Phe(NO2)-Ser-Phe | Plasmodium falciparum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 6 | pH 3.5: about 75% of maximal activity, pH 6.0: about 40% of maximal activity, hydrolysis of Leu-Glu-Arg-Ile-Phe-Phe(NO2)-Ser-Phe | Plasmodium falciparum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Ki-value for Ro17-7109 is above 0.05 mM | Plasmodium falciparum | |
0.000008 | - |
Ro40-5576 | - |
Plasmodium falciparum | |
0.000009 | - |
Ro40-4388 | - |
Plasmodium falciparum | |
0.0003 | - |
N'1-[(E)-(2,3-dihydroxyphenyl)methylidene]-N'3-[(Z)-(2,3-dihydroxyphenyl)methylidene]benzene-1,3-dicarbohydrazide | - |
Plasmodium falciparum |