Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N'1-[(E)-(2,3-dihydroxyphenyl)methylidene]-N'3-[(Z)-(2,3-dihydroxyphenyl)methylidene]benzene-1,3-dicarbohydrazide | - |
Plasmodium falciparum | |
Ro40-4388 | - |
Plasmodium falciparum | |
Ro40-5576 | - |
Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
Leu-Glu-Arg-Ile-Phe-Phe(NO2)-Ser-Phe | pH 4.4, 37°C | Plasmodium falciparum | |
0.03 | - |
Leu-Glu-Arg-Val-Phe-Phe(NO2)-Ser-Phe | pH 4.4, 37°C | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Hemoglobin + H2O | Plasmodium falciparum | during the infection of erythrocytes by the human malaria parasite Plasmodium falciparum, up to 80% of the host cell hemoglobin is degraded to provide amino acids for parasite nutrition. Plasmepsin I and plasmepsin II are believed to be responsible for initiating the catabolic process | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Hemoglobin + H2O | during the infection of erythrocytes by the human malaria parasite Plasmodium falciparum, up to 80% of the host cell hemoglobin is degraded to provide amino acids for parasite nutrition. Plasmepsin I and plasmepsin II are believed to be responsible for initiating the catabolic process | Plasmodium falciparum | ? | - |
? | |
Leu-Glu-Arg-Ile-Phe-Phe(NO2)-Ser-Phe + H2O | - |
Plasmodium falciparum | Leu-Glu-Arg-Ile-Phe + Phe(NO2)-Ser-Phe | - |
? | |
Leu-Glu-Arg-Val-Phe-Phe(NO2)-Ser-Phe + H2O | - |
Plasmodium falciparum | Leu-Glu-Arg-Val-Phe + Phe(NO2)-Ser-Phe | - |
? | |
additional information | plasmepsin I is 5 to 9times less effective than plasmepsin II at cleaving substrates which mimic the proposed initial cleavage site in the alpha-chain of hemoglobin | Plasmodium falciparum | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
Leu-Glu-Arg-Ile-Phe-Phe(NO2)-Ser-Phe | pH 4.4, 37°C | Plasmodium falciparum | |
1.6 | - |
Leu-Glu-Arg-Val-Phe-Phe(NO2)-Ser-Phe | pH 4.4, 37°C | Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Plasmodium falciparum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 7 | active over the range | Plasmodium falciparum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000004 | - |
Ro40-4388 | pH 4.4, 37°C | Plasmodium falciparum | |
0.000006 | - |
Ro40-5576 | pH 4.4 | Plasmodium falciparum | |
0.0003 | - |
N'1-[(E)-(2,3-dihydroxyphenyl)methylidene]-N'3-[(Z)-(2,3-dihydroxyphenyl)methylidene]benzene-1,3-dicarbohydrazide | pH 4.4 | Plasmodium falciparum |