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Literature summary for 3.4.23.38 extracted from

  • Tyas, L.; Moon, R.P.; Loetscher, H.; Dunn, B.M.; Kay, J.; Ridley, R.G.; Berry, C.
    Plasmepsins I and II from the malarial parasite Plasmodium falciparum (1998), Adv. Exp. Med. Biol., 436, 407-411.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
N'1-[(E)-(2,3-dihydroxyphenyl)methylidene]-N'3-[(Z)-(2,3-dihydroxyphenyl)methylidene]benzene-1,3-dicarbohydrazide
-
Plasmodium falciparum
Ro40-4388
-
Plasmodium falciparum
Ro40-5576
-
Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
Leu-Glu-Arg-Ile-Phe-Phe(NO2)-Ser-Phe pH 4.4, 37°C Plasmodium falciparum
0.03
-
Leu-Glu-Arg-Val-Phe-Phe(NO2)-Ser-Phe pH 4.4, 37°C Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Hemoglobin + H2O Plasmodium falciparum during the infection of erythrocytes by the human malaria parasite Plasmodium falciparum, up to 80% of the host cell hemoglobin is degraded to provide amino acids for parasite nutrition. Plasmepsin I and plasmepsin II are believed to be responsible for initiating the catabolic process ?
-
?

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Hemoglobin + H2O during the infection of erythrocytes by the human malaria parasite Plasmodium falciparum, up to 80% of the host cell hemoglobin is degraded to provide amino acids for parasite nutrition. Plasmepsin I and plasmepsin II are believed to be responsible for initiating the catabolic process Plasmodium falciparum ?
-
?
Leu-Glu-Arg-Ile-Phe-Phe(NO2)-Ser-Phe + H2O
-
Plasmodium falciparum Leu-Glu-Arg-Ile-Phe + Phe(NO2)-Ser-Phe
-
?
Leu-Glu-Arg-Val-Phe-Phe(NO2)-Ser-Phe + H2O
-
Plasmodium falciparum Leu-Glu-Arg-Val-Phe + Phe(NO2)-Ser-Phe
-
?
additional information plasmepsin I is 5 to 9times less effective than plasmepsin II at cleaving substrates which mimic the proposed initial cleavage site in the alpha-chain of hemoglobin Plasmodium falciparum ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3
-
Leu-Glu-Arg-Ile-Phe-Phe(NO2)-Ser-Phe pH 4.4, 37°C Plasmodium falciparum
1.6
-
Leu-Glu-Arg-Val-Phe-Phe(NO2)-Ser-Phe pH 4.4, 37°C Plasmodium falciparum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Plasmodium falciparum

pH Range

pH Minimum pH Maximum Comment Organism
3 7 active over the range Plasmodium falciparum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000004
-
Ro40-4388 pH 4.4, 37°C Plasmodium falciparum
0.000006
-
Ro40-5576 pH 4.4 Plasmodium falciparum
0.0003
-
N'1-[(E)-(2,3-dihydroxyphenyl)methylidene]-N'3-[(Z)-(2,3-dihydroxyphenyl)methylidene]benzene-1,3-dicarbohydrazide pH 4.4 Plasmodium falciparum