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Literature summary for 3.4.23.35 extracted from

  • MacKay, V.L.
    Barrierpepsin (2004), Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. ), 1, 126-128.
No PubMed abstract available
Search for more literature for 3.4.23.35

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by pepstatin A, EDTA, alpha-amincaproic acid, and several serine proteinase inhibitors, e.g. tosyl arginyl methyl ester Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted by the secretory pathway in active form Saccharomyces cerevisiae
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement for divalent cations Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
200000
-
 x * 200000, SDS-PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
WHWLQLKQGQPMY + H2O Saccharomyces cerevisiae absolutely specific for the single neutral substrate peptide pheromone alpha-factor WHWLQL + KQGQPMY
-
 ?
WHWLQLKQGQPMY + H2O Saccharomyces cerevisiae X2180-1A absolutely specific for the single neutral substrate peptide pheromone alpha-factor WHWLQL + KQGQPMY
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 gene BAR1
-
Saccharomyces cerevisiae X2180-1A
-
 gene BAR1
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein highly glycosylated enzyme Saccharomyces cerevisiae

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme 2000fold by two different steps of anion exchange chromatography and gle filtration Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
WHWLQLKQGQPMY + H2O absolutely specific for the single neutral substrate peptide pheromone alpha-factor Saccharomyces cerevisiae WHWLQL + KQGQPMY
-
 ?
WHWLQLKQGQPMY + H2O absolutely specific for the single neutral substrate peptide pheromone alpha-factor, commercial substrate preparation Saccharomyces cerevisiae WHWLQL + KQGQPMY
-
 ?
WHWLQLKQGQPMY + H2O absolutely specific for the single neutral substrate peptide pheromone alpha-factor Saccharomyces cerevisiae X2180-1A WHWLQL + KQGQPMY
-
 ?
WHWLQLKQGQPMY + H2O absolutely specific for the single neutral substrate peptide pheromone alpha-factor, commercial substrate preparation Saccharomyces cerevisiae X2180-1A WHWLQL + KQGQPMY
-
 ?

Subunits

Subunits Comment Organism
? x * 200000, SDS-PAGE Saccharomyces cerevisiae
More primary sequence analysis Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
Bar proteinase
-
 Saccharomyces cerevisiae
Barrier proteinase
-
 Saccharomyces cerevisiae
More the enzyme belongs to the A1 peptidase family Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 5.3
-
 Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
2.6 6.8 50% of maximal activity at pH 2.6 and pH 6.8 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
additional information no requirement for cofactors Saccharomyces cerevisiae