Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.23.34 extracted from

  • Cappiello, M.G.; Wu, Z.; Scott, B.B.; McGeehan, G.M.; Harrison, R.K.
    Purification and characterization of recombinant human cathepsin E expressed in human kidney cell line 293 (2004), Protein Expr. Purif., 37, 53-60.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
HEK-293 cells are transfected with the full length human cathepsin E gene cloned into pcDNA3.1V5His Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
pepstatin A
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0068
-
MOCAc-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH2 25°C, pH 4.0 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46343
-
x * 46343, mass spectral analysis Homo sapiens
92000
-
dimer due to dimeric linkage between two monomers, non-denaturing PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(7-methoxycoumarin-4-yl)acetyl-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH2 + H2O
-
Homo sapiens (7-methoxycoumarin-4-yl)acetyl-Gly-Lys-Pro-Ile-Leu-Phe + Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH2
-
?

Subunits

Subunits Comment Organism
? x * 46343, mass spectral analysis Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
360
-
MOCAc-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH2 25°C, pH 4.0 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5 4.5
-
Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
3.5 4.8 pH 3.5.-4.5: optimum, pH 4.8: about 25% of maximal activity Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000002
-
pepstatin A 25°C, pH 4.0 Homo sapiens