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Literature summary for 3.4.23.34 extracted from

  • Yasuda, Y.; Tsukuba, T.; Okamoto, K.; Kadowaki, T.; Yamamoto, K.
    The role of the cathepsin E propeptide in correct folding, maturation and sorting to the endosome (2005), J. Biochem., 138, 621-630.
    View publication on PubMed
Search for more literature for 3.4.23.34

Cloned(Commentary)

Cloned (Comment) Organism
construction of two fusion proteins using chimeric DNAs encoding the cathepsin E propeptide fused to the mature cathepsin D tagged with HA at the COOH terminus and encoding the cathepsin D propeptide fused to the mature cathepsin E Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
additional information construction of two fusion proteins using chimeric DNAs encoding the cathepsin E propeptide fused to the mature cathepsin D tagged with HA at the COOH terminus and encoding the cathepsin D propeptide fused to the mature cathepsin E Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Ascaris pepsin inhibitor
-
 Rattus norvegicus
pepstatin A
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00153
-
 (7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(Dnp)-D-Arg-NH2 40°C, pH 4.0, wild-type cathepsin E Rattus norvegicus
0.0028
-
 (7-methoxycoumarin-4-yl)acetyl-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH2 40°C, pH 4.0, wild-type cathepsin E Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification propeptide of cathepsin E is essential for the correct folding, maturation, and targeting of this protein to its final destination Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(7-methoxycoumarin-4-yl)acetyl-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH2 + H2O
-
 Rattus norvegicus ?
-
 ?
(7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(Dnp)-D-Arg-NH2 + H2O
-
 Rattus norvegicus ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.52
-
 (7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(Dnp)-D-Arg-NH2 40°C, pH 4.0, wild-type cathepsin E Rattus norvegicus
13.58
-
 (7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(Dnp)-D-Arg-NH2 40°C, pH 4.0, wild-type cathepsin E Rattus norvegicus
32.2
-
 (7-methoxycoumarin-4-yl)acetyl-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH2 40°C, pH 4.0, wild-type cathepsin E Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000031
-
 pepstatin A 40°C, pH 4.0, wild-type cathepsin E Rattus norvegicus
0.0000135
-
 Ascaris pepsin inhibitor 40°C, pH 4.0, wild-type cathepsin E Rattus norvegicus