Cloned (Comment) | Organism |
---|---|
expressed in Chinese hamster ovary cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-tert-Butoxycarbonyl-His-Pro-Phe-His-4-amino-3-hydroxy-6-methylheptanoic acid-Leu-Phe-NH2 | - |
Homo sapiens | |
pepstatin | also inhibits conversion of procathepsin E to cathepsin E | Homo sapiens | |
Pro-Thr-Glu-Phe-PSI(CH2-NH)-Nle-Arg-Leu | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | recombinant enzyme is catalytically indistinguishable from natural cathepsin | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | recombinant enzyme | Homo sapiens | 5737 | - |
endoplasmic reticulum | recombinant enzyme | Homo sapiens | 5783 | - |
soluble | - |
Homo sapiens | - |
- |
vacuole | recombinant enzyme | Homo sapiens | 5773 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
82000 | - |
human, SDS-PAGE, non-reducing conditions | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | enzymes: N-glycosylated with high-mannose type oligosaccharide chain | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O | - |
Homo sapiens | Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu | - |
? | |
Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu + H2O | synthetic chromogenic peptide | Homo sapiens | Pro-Pro-Thr-Ile-Phe + (4-nitro)Phe-Arg-Leu | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
20 min, inactivation of native enzyme at pH 5.5, only 10% loss of recombinant enzyme activity | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | activation | Homo sapiens | |
ATP | activates native, not recombinant enzyme | Homo sapiens |