Literature summary for 3.4.23.34 extracted from

Hill, J.; Montgomery, D.S.; Kay, J.
Human cathepsin E produced in E. coli (1993), FEBS Lett., 326, 101-104.

Search for more literature for 3.4.23.34

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3)pLysS	Homo sapiens
human	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
(D)-His-Pro-Phe-His-Leu-PSI(CH2-NH)-Leu-Val-Tyr	i.e. H-77, with reduced isostere as replacement of the-CO-NH- of the peptide bond	Homo sapiens
pepstatin	-	Homo sapiens
Protein inhibitor from Ascaris lumbricoides	MW 17000	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.065	-	Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu	-	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
inclusion body	recombinant enzyme in Escherichia coli	Homo sapiens	16234	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
76000	-	human, recombinant enzyme, SDS-PAGE, non-reducing conditions	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
gastric adenocarcinoma cell	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Hemoglobin + H2O	-	Homo sapiens	Hydrolyzed hemoglobin	-	?
Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O	-	Homo sapiens	Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu	-	?
Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu + H2O	-	Homo sapiens	Pro-Pro-Thr-Ile-Phe + (4-nitro)Phe-Arg-Leu	-	?

Subunits

Subunits	Comment	Organism
dimer	recombinant enzyme, SDS-PAGE, reducing conditions	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
115	-	Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu	-	Homo sapiens
132	-	Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu	-	Homo sapiens

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Release 2023.1 (January 2023)