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Literature summary for 3.4.23.34 extracted from

  • Tsukuba, T.; Hori, H.; Azuma, T.; Takahashi, T.; Taggart, R.T.; Akamine, A.; Ezaki, M.; Nakanishi, H.; Sakai, H.; Yamamoto, K.
    Isolation and characterization of recombinant human cathepsin E expressed in Chinese hamster ovary cells (1993), J. Biol. Chem., 268, 7276-7282.
    View publication on PubMed
Search for more literature for 3.4.23.34

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Chinese hamster ovary cells Homo sapiens
human Homo sapiens
procathepsin E Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
pepstatin hemoglobin Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information apparent Km-values of hemoglobin hydrolysis Homo sapiens
0.06
-
 Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu
-
 Homo sapiens
0.08
-
 Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu
-
 Homo sapiens
0.09 0.4 Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu recombinant enzyme forms Homo sapiens
0.1 0.11 Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu recombinant enzyme form Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm recombinant enzyme Homo sapiens 5737
-
endoplasmic reticulum recombinant enzyme Homo sapiens 5783
-
additional information immunocytochemical localization of recombinant enzyme in transfected cells Homo sapiens
-
-
soluble
-
 Homo sapiens
-
-
vacuole recombinant enzyme Homo sapiens 5773
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82000
-
 human, SDS-PAGE, non-reducing conditions Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein enzymes: N-glycosylated with high-mannose type oligosaccharide chain Homo sapiens
glycoprotein human recombinant enzyme Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant from heterologous Chinese hamster ovary cells (3 forms: cytosolic s-CE and vacuolar v-CE-1 and 2) Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Hemoglobin + H2O
-
 Homo sapiens Hydrolyzed hemoglobin
-
 ?
Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O
-
 Homo sapiens Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu
-
 ?
Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu + H2O
-
 Homo sapiens Pro-Pro-Thr-Ile-Phe + (4-nitro)Phe-Arg-Leu
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 assay at Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 no difference in thermostability between recombinant and native enzyme forms Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 no difference with respect to pH-dependence of hydrolysis between recombinant and native enzyme Homo sapiens