Cloned (Comment) | Organism |
---|---|
guinea pig | Cavia porcellus |
procathepsin E | Cavia porcellus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
pepstatin | - |
Cavia porcellus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
2 * 39000, SDS-PAGE, reducing conditions | Cavia porcellus |
40086 | - |
2 * 40086, procathepsin E, deduced from nucleotide sequence | Cavia porcellus |
80000 | - |
guinea pig, procathepsin E, gel filtration, SDS-PAGE, non-reducing conditions | Cavia porcellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cavia porcellus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 4% w/w content of proenzyme | Cavia porcellus |
Purification (Comment) | Organism |
---|---|
- |
Cavia porcellus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
gastric mucosa | - |
Cavia porcellus | - |
stomach | - |
Cavia porcellus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Cavia porcellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Bovine serum albumin + H2O | pH 5, at 3.6% (dimeric enzyme) or 4.1% (monomeric enzyme) the rate of hemoglobin hydrolysis | Cavia porcellus | Hydrolyzed bovine serum albumin | - |
? | |
casein + H2O | pH 5, at 9.1% (dimeric enzyme) or 8.9% (monomeric enzyme) the rate of hemoglobin hydrolysis | Cavia porcellus | hydrolyzed casein | - |
? | |
Cytochrome c + H2O | pH 5, at 2.5% (dimeric enzyme) or 2.3% (monomeric enzyme) the rate of hemoglobin hydrolysis | Cavia porcellus | Hydrolyzed cytochrome c | - |
? | |
Egg albumin + H2O | pH 5, at 1% (dimeric enzyme) or 1.1% (monomeric enzyme) the rate of hemoglobin hydrolysis | Cavia porcellus | Hydrolyzed egg albumin | - |
? | |
Hemoglobin + H2O | preferred substrate | Cavia porcellus | Hydrolyzed hemoglobin | - |
? | |
Hemoglobin + H2O | at pH 2 the two catalytically active subunits have the same activity vs. hemoglobin, but at pH 5 they have a slightly higher activity than the enzyme dimer | Cavia porcellus | Hydrolyzed hemoglobin | - |
? | |
Human gamma-globulin + H2O | pH 5, at 0.5% (dimeric enzyme) or 0.6% (monomeric enzyme) the rate of hemoglobin hydrolysis | Cavia porcellus | Hydrolyzed human gamma-globulin | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | procathepsin E, SDS-PAGE, reducing conditions | Cavia porcellus |
dimer | 2 * 39000, SDS-PAGE, reducing conditions | Cavia porcellus |
dimer | 2 * 40086, procathepsin E, deduced from nucleotide sequence | Cavia porcellus |
More | interconversion between dimeric and monomeric form of enzyme and proenzyme is reversible and regulated by low concentrations of reducing agents | Cavia porcellus |
More | dimeric form is maintained throughout activation of procathepsin E to cathepsin E | Cavia porcellus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
2 h, enzyme dimer: 60% loss of activity at pH 4, 45% at pH 5, 20% at pH 6 and pH 8.8, stable at pH 7, 10% loss of activity at pH 8, enzyme monomer: 65% loss of activity at pH 4, 55% at pH 5, 20% at pH 6, 10% at pH 7, 50% at pH 7.5, 70% at pH 8 and 95% at pH 8.8 | Cavia porcellus |
37 | - |
6 h at pH 6-7.4, rather stable | Cavia porcellus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2.5 | - |
approximate value, hemoglobin as substrate | Cavia porcellus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
1 | 4 | 83% of maximal activity at pH 1 and 55% of maximal activity at pH 4, hemoglobin as substrate | Cavia porcellus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
dimer is stable at weakly alkaline pH while monomer rapidly loses activity above pH 7 | Cavia porcellus |
4 | - |
2 h at 28°C, 60-65% loss of activity | Cavia porcellus |
5 | - |
2 h at 28°C, 45% (dimer) and 55% (monomer) loss of activity | Cavia porcellus |
6 | - |
2 h at 28°C, 20% loss of activity | Cavia porcellus |
7 | - |
2 h at 28°C, stable (dimer), 10% loss of activity (monomer) | Cavia porcellus |
7.5 | - |
2 h at 28°C, 50% loss of monomeric enzyme activity | Cavia porcellus |
8 | - |
2 h at 28°C, 10% (dimer) and 70% (monomer) loss of activity | Cavia porcellus |
8.8 | - |
2 h at 28°C, 20% (dimer) and 95% (monomer) loss of activity | Cavia porcellus |