Activating Compound | Comment | Organism | Structure |
---|---|---|---|
GTP | activation, maintains enzyme in its active conformation at pH 5 and above | Homo sapiens | |
additional information | or 2-mercaptoethanol | Homo sapiens |
General Stability | Organism |
---|---|
ATP, CTP or GTP stabilizes in the range of pH 5-7.4, not below pH 5, adenosine, sodium triphosphate, ADP, 2,3-diphosphoglycerate do not stabilize | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | 2-mercaptoethanol, Mg2+ or vanadate | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no activation by Mg2+ or vanadate | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
gastric mucosa | - |
Homo sapiens | - |
stomach | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
casein + H2O | - |
Homo sapiens | hydrolyzed casein | - |
? | |
Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O | - |
Homo sapiens | Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu | - |
? | |
Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu + H2O | synthetic chromogenic peptide | Homo sapiens | Pro-Pro-Thr-Ile-Phe + (4-nitro)Phe-Arg-Leu | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | pH-dependence of kinetic constants | Homo sapiens | |
5 | - |
Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu | value below, with ATP, pH 7 | Homo sapiens | |
11 | - |
Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu | with ATP, pH 6.6 and without ATP, pH 5 | Homo sapiens | |
16 | - |
Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu | with ATP, pH 6.2 | Homo sapiens | |
26 | - |
Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu | without ATP, pH 5.4 | Homo sapiens | |
31 | - |
Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu | with ATP, pH 5 | Homo sapiens | |
36 | - |
Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu | without ATP, pH 4.5 | Homo sapiens | |
39 | - |
Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu | with ATP, pH 4.5 and pH 5.4 | Homo sapiens | |
40 | - |
Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu | with ATP, pH 5.8 | Homo sapiens | |
47 | - |
Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu | without ATP, pH 5 | Homo sapiens | |
54 | - |
Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu | without ATP, pH 5 | Homo sapiens | |
56 | - |
Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu | with ATP, pH 5.8 | Homo sapiens | |
72 | - |
Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu | with ATP, pH 5.4 | Homo sapiens | |
76 | - |
Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu | without ATP, pH 4.5 | Homo sapiens | |
77 | - |
Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu | with ATP, pH 4.5 and pH 5 | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | active in the presence of ATP | Homo sapiens |
5.8 | - |
and above, no activity in the absence of ATP | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | activation | Homo sapiens | |
ATP | maintains enzyme in its active conformation at pH 5 and above | Homo sapiens | |
ATP | no activation at low pH-values, e.g. pH 4.5 | Homo sapiens | |
CTP | activation, maintains enzyme in its active conformation at pH 5 and above | Homo sapiens |