Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.23.34 extracted from

  • Jupp, R.A.; Richards, A.D.; Kay, J.; Dunn, B.M.; Wyckoff, J.B.; Samloff, M.; Yamamoto, K.
    Identification of the aspartic proteinases from human erythrocyte membranes and gastric mucosa (slow-moving proteinase) as catalytically equivalent to cathepsin E (1988), Biochem. J., 254, 895-898.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.23.34

Inhibitors

Inhibitors Comment Organism Structure
(D)-His-Pro-Phe-His-Leu-PSI(CH2-NH)-Leu-Val-Tyr i.e. H-77, with reduced isostere as replacement of the-CO-NH- of the peptide bond Homo sapiens
(D)-His-Pro-Phe-His-Leu-PSI(CH2-NH)-Leu-Val-Tyr i.e. H-77, with reduced isostere as replacement of the-CO-NH- of the peptide bond Rattus norvegicus
N-tert-Butoxycarbonyl-His-Pro-Phe-His-4-amino-3-hydroxy-6-methylheptanoic acid-Leu-Phe-NH2
-
 Homo sapiens
N-tert-Butoxycarbonyl-His-Pro-Phe-His-4-amino-3-hydroxy-6-methylheptanoic acid-Leu-Phe-NH2
-
 Rattus norvegicus
N-tert-Butoxycarbonyl-His-Pro-Phe-His-Leu-PSI(CHOH-CH2)-Val-Ile-His i.e. H-261, with reduced isostere as replacement of the -CO-NH- of the peptide bond Homo sapiens
N-tert-Butoxycarbonyl-His-Pro-Phe-His-Leu-PSI(CHOH-CH2)-Val-Ile-His i.e. H-261, with reduced isostere as replacement of the -CO-NH- of the peptide bond Rattus norvegicus
pepstatin
-
 Homo sapiens
pepstatin
-
 Oryctolagus cuniculus
pepstatin
-
 Rattus norvegicus
Pro-Thr-Glu-Phe-PSI(CH2-NH)-Nle-Arg-Leu i.e. H-297, with reduced isostere as replacement of the -CO-NH- of the peptide bond Homo sapiens
Pro-Thr-Glu-Phe-PSI(CH2-NH)-Nle-Arg-Leu i.e. H-297, with reduced isostere as replacement of the -CO-NH- of the peptide bond Rattus norvegicus
Pro-Thr-Glu-Phe-PSI(CH2-NH)-Phe-Arg-Glu i.e. H-256, with reduced isostere as replacement of the -CO-NH- of the peptide bond Homo sapiens
Pro-Thr-Glu-Phe-PSI(CH2-NH)-Phe-Arg-Glu i.e. H-256, with reduced isostere as replacement of the -CO-NH- of the peptide bond Rattus norvegicus
Protein inhibitor from Ascaris lumbricoides MW 17000 Homo sapiens
Protein inhibitor from Ascaris lumbricoides MW 17000 Oryctolagus cuniculus
Protein inhibitor from Ascaris lumbricoides MW 17000 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information pH-dependence of kinetic constants Homo sapiens
additional information
-
 additional information pH-dependence of kinetic constants Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 2 * 42000, about, SDS-PAGE, reducing conditions Homo sapiens
42000
-
 2 * 42000, about, SDS-PAGE, reducing conditions Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Oryctolagus cuniculus
-
-
-
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
 Homo sapiens
-
erythrocyte
-
 Rattus norvegicus
-
gastric mucosa
-
 Homo sapiens
-
gastric mucosa
-
 Rattus norvegicus
-
gastric mucosa
-
 Oryctolagus cuniculus
-
spleen
-
 Rattus norvegicus
-
stomach
-
 Homo sapiens
-
stomach
-
 Rattus norvegicus
-
stomach
-
 Oryctolagus cuniculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O
-
 Homo sapiens Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu
-
 ?
Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O
-
 Rattus norvegicus Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu
-
 ?
Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O
-
 Oryctolagus cuniculus Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu
-
 ?
Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu + H2O synthetic chromogenic peptide Homo sapiens Pro-Pro-Thr-Ile-Phe + (4-nitro)Phe-Arg-Leu
-
 ?
Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu + H2O synthetic chromogenic peptide Rattus norvegicus Pro-Pro-Thr-Ile-Phe + (4-nitro)Phe-Arg-Leu
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Oryctolagus cuniculus
dimer 2 * 42000, about, SDS-PAGE, reducing conditions Homo sapiens
dimer 2 * 42000, about, SDS-PAGE, reducing conditions Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens
37
-
 assay at Rattus norvegicus
37
-
 assay at Oryctolagus cuniculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information pH-dependence of kinetic constants Homo sapiens
additional information
-
 additional information pH-dependence of kinetic constants Rattus norvegicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.8
-
 75% loss of activity of erythrocyte membrane aspartic proteinase from human erythrocytes, inactivation of slow-moving proteinase from human gastric mucosa and cathepsin E from rat spleen, instable above pH 5.8 Homo sapiens
5.8
-
 75% loss of activity of erythrocyte membrane aspartic proteinase from human erythrocytes, inactivation of slow-moving proteinase from human gastric mucosa and cathepsin E from rat spleen, instable above pH 5.8 Rattus norvegicus