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Literature summary for 3.4.23.32 extracted from

  • Kondo, M.Y.; Okamoto, D.N.; Santos, J.A.; Juliano, M.A.; Oda, K.; Pillai, B.; James, M.N.; Juliano, L.; Gouvea, I.E.
    Studies on the catalytic mechanism of a glutamic peptidase (2010), J. Biol. Chem., 285, 21437-21445.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.23.32

Inhibitors

Inhibitors Comment Organism Structure
acetyl-Phe--Lys-Phe-(3S,4S)-phenylstatinyl-Leu-Arg-NH2 SGP transition state mimic inhibitor Scytalidium lignicola
NaCl a substantial decrease of the SGP catalytic efficiency (kcat/Km) is observed as the NaCl concentration is increases up to 1 M, while in the range up to 200 mM NaCl the effect is mainly in Km because there is no significant decrease in kcat values. A significant decrease in the kcat values is observed as the salt concentration is further increased from 200 to 800 mM NaCl Scytalidium lignicola

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0001
-
 2-aminobenzoyl-KLHPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
0.0002
-
 2-aminobenzoyl-KLFPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
0.00025
-
 2-aminobenzoyl-KLYPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
0.00026
-
 2-aminobenzoyl-KLNPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
0.0003
-
 2-aminobenzoyl-KLRPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
0.0004
-
 2-aminobenzoyl-KLHSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
0.0004
-
 2-aminobenzoyl-KLPSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
0.0004
-
 2-aminobenzoyl-KLSSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
0.0005
-
 2-aminobenzoyl-KLKSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
0.00055
-
 2-aminobenzoyl-KLKPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
0.0008
-
 2-aminobenzoyl-KLMSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
0.0008
-
 2-aminobenzoyl-KLRSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
0.0008
-
 2-aminobenzoyl-KLQSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
0.0008
-
 2-aminobenzoyl-KLESSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
0.0009
-
 2-aminobenzoyl-KLQPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
0.0011
-
 2-aminobenzoyl-KLYSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
0.0012
-
 2-aminobenzoyl-KLFSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
0.0014
-
 2-aminobenzoyl-KLSPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
0.0016
-
 2-aminobenzoyl-KLLSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
0.0017
-
 2-aminobenzoyl-KLVSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
0.002
-
 2-aminobenzoyl-KLGPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
0.0022
-
 2-aminobenzoyl-KLAPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
0.0023
-
 2-aminobenzoyl-KLASSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
0.003
-
 2-aminobenzoyl-KLNSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
0.0039
-
 2-aminobenzoyl-KLDPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
0.004
-
 2-aminobenzoyl-KLESPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
0.0046
-
 2-aminobenzoyl-KLDSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
0.0046
-
 2-aminobenzoyl-KLGSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola

Organism

Organism UniProt Comment Textmining
Scytalidium lignicola
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminobenzoyl-KLAPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
 Scytalidium lignicola 2-aminobenzoyl-KLA + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
 ?
2-aminobenzoyl-KLASSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
 Scytalidium lignicola 2-aminobenzoyl-KLA + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
 ?
2-aminobenzoyl-KLDPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
 Scytalidium lignicola 2-aminobenzoyl-KLD + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
 ?
2-aminobenzoyl-KLDSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
 Scytalidium lignicola 2-aminobenzoyl-KLD + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
 ?
2-aminobenzoyl-KLEPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
 Scytalidium lignicola 2-aminobenzoyl-KLE + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
 ?
2-aminobenzoyl-KLESPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
 Scytalidium lignicola 2-aminobenzoyl-KLES + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
 ?
2-aminobenzoyl-KLESSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
 Scytalidium lignicola 2-aminobenzoyl-KLE + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
 ?
2-aminobenzoyl-KLFPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
 Scytalidium lignicola 2-aminobenzoyl-KLF + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
 ?
2-aminobenzoyl-KLFSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
 Scytalidium lignicola 2-aminobenzoyl-KLF + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
 ?
2-aminobenzoyl-KLGPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
 Scytalidium lignicola 2-aminobenzoyl-KLG + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
 ?
2-aminobenzoyl-KLGSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
 Scytalidium lignicola 2-aminobenzoyl-KLG + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
 ?
2-aminobenzoyl-KLHPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
 Scytalidium lignicola 2-aminobenzoyl-KLH + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
 ?
2-aminobenzoyl-KLHSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
 Scytalidium lignicola 2-aminobenzoyl-KLH + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
 ?
2-aminobenzoyl-KLKPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
 Scytalidium lignicola 2-aminobenzoyl-KLK + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
 ?
2-aminobenzoyl-KLKSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
 Scytalidium lignicola 2-aminobenzoyl-KLK + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
 ?
2-aminobenzoyl-KLLPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
 Scytalidium lignicola 2-aminobenzoyl-KLL + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
 ?
2-aminobenzoyl-KLLSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
 Scytalidium lignicola 2-aminobenzoyl-KLL + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
 ?
2-aminobenzoyl-KLMPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
 Scytalidium lignicola 2-aminobenzoyl-KLM + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
 ?
2-aminobenzoyl-KLMSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
 Scytalidium lignicola 2-aminobenzoyl-KLM + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
 ?
2-aminobenzoyl-KLNPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
 Scytalidium lignicola 2-aminobenzoyl-KLN + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
 ?
2-aminobenzoyl-KLNSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
 Scytalidium lignicola 2-aminobenzoyl-KLN + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
 ?
2-aminobenzoyl-KLPPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
 Scytalidium lignicola 2-aminobenzoyl-KLP + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
 ?
2-aminobenzoyl-KLPSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
 Scytalidium lignicola 2-aminobenzoyl-KLP + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
 ?
2-aminobenzoyl-KLQPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
 Scytalidium lignicola 2-aminobenzoyl-KLQ + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
 ?
2-aminobenzoyl-KLQSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
 Scytalidium lignicola 2-aminobenzoyl-KLQ + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
 ?
2-aminobenzoyl-KLRPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
 Scytalidium lignicola 2-aminobenzoyl-KLR + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
 ?
2-aminobenzoyl-KLRSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
 Scytalidium lignicola 2-aminobenzoyl-KLR + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
 ?
2-aminobenzoyl-KLSPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
 Scytalidium lignicola 2-aminobenzoyl-KLS + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
 ?
2-aminobenzoyl-KLSSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
 Scytalidium lignicola 2-aminobenzoyl-KLS + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
 ?
2-aminobenzoyl-KLVPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
 Scytalidium lignicola 2-aminobenzoyl-KLV + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
 ?
2-aminobenzoyl-KLVSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
 Scytalidium lignicola 2-aminobenzoyl-KLV + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
 ?
2-aminobenzoyl-KLYPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
 Scytalidium lignicola 2-aminobenzoyl-KLY + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
 ?
2-aminobenzoyl-KLYSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
 Scytalidium lignicola 2-aminobenzoyl-KLY + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
 ?
additional information the enzyme shows preference for Phe and His over Leu, Ile, Val, Arg, and Lys. Peptides with positively charged amino acids Arg and Lys or aliphatic amino acids Leu, Ile, and Val are all poor substrates, 2-aminobenzoyl-KLIPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine], 2-aminobenzoyl-KLWPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine], 2-aminobenzoyl-KLVSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine], 2-aminobenzoyl-KLISSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine], 2-aminobenzoyl-KLWSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine], and 2-aminobenzoyl-KLPSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] are resistant to hydrolysis Scytalidium lignicola ?
-
 ?

Synonyms

Synonyms Comment Organism
scytalidoglutamic peptidase
-
 Scytalidium lignicola
SGP
-
 Scytalidium lignicola

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03
-
 2-aminobenzoyl-KLSPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
0.048
-
 2-aminobenzoyl-KLVSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
0.063
-
 2-aminobenzoyl-KLSSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
0.2
-
 2-aminobenzoyl-KLESSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
0.4
-
 2-aminobenzoyl-KLQPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
0.4
-
 2-aminobenzoyl-KLGPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
0.4
-
 2-aminobenzoyl-KLESPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
0.6
-
 2-aminobenzoyl-KLDPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
0.8
-
 2-aminobenzoyl-KLQSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
1.1
-
 2-aminobenzoyl-KLRPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
1.6
-
 2-aminobenzoyl-KLAPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
3.5
-
 2-aminobenzoyl-KLKPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
3.6
-
 2-aminobenzoyl-KLGSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
6
-
 2-aminobenzoyl-KLASSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
6.5
-
 2-aminobenzoyl-KLNPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
7.7
-
 2-aminobenzoyl-KLPSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
7.7
-
 2-aminobenzoyl-KLLSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
8
-
 2-aminobenzoyl-KLKSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
8.7
-
 2-aminobenzoyl-KLYPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
13
-
 2-aminobenzoyl-KLFPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
15
-
 2-aminobenzoyl-KLRSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
17
-
 2-aminobenzoyl-KLDSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
33
-
 2-aminobenzoyl-KLHPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
86
-
 2-aminobenzoyl-KLYSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
105
-
 2-aminobenzoyl-KLMSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
145
-
 2-aminobenzoyl-KLHSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
198
-
 2-aminobenzoyl-KLNSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
200
-
 2-aminobenzoyl-KLFSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Scytalidium lignicola

pH Stability

pH Stability pH Stability Maximum Comment Organism
8 9.5 in the pH range 8.0-9.5, the enzyme becomes irreversibly inactivated Scytalidium lignicola

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
20
-
 2-aminobenzoyl-KLSPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
30
-
 2-aminobenzoyl-KLVSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
100
-
 2-aminobenzoyl-KLESPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
150
-
 2-aminobenzoyl-KLDPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
160
-
 2-aminobenzoyl-KLSSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
200
-
 2-aminobenzoyl-KLGPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
250
-
 2-aminobenzoyl-KLESSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
400
-
 2-aminobenzoyl-KLQPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
700
-
 2-aminobenzoyl-KLAPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
800
-
 2-aminobenzoyl-KLGSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
1000
-
 2-aminobenzoyl-KLQSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
2600
-
 2-aminobenzoyl-KLASSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
3700
-
 2-aminobenzoyl-KLDSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
3700
-
 2-aminobenzoyl-KLRPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
4800
-
 2-aminobenzoyl-KLLSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
6400
-
 2-aminobenzoyl-KLKPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
16000
-
 2-aminobenzoyl-KLKSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
19000
-
 2-aminobenzoyl-KLRSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
19000
-
 2-aminobenzoyl-KLPSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
26000
-
 2-aminobenzoyl-KLNPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
35000
-
 2-aminobenzoyl-KLYPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
65000
-
 2-aminobenzoyl-KLFPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
66000
-
 2-aminobenzoyl-KLNSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
78000
-
 2-aminobenzoyl-KLYSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
131000
-
 2-aminobenzoyl-KLMSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
167000
-
 2-aminobenzoyl-KLFSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
330000
-
 2-aminobenzoyl-KLHPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola
363000
-
 2-aminobenzoyl-KLHSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] in 100 mM sodium acetate buffer (pH 4.5) at 37°C Scytalidium lignicola