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Literature summary for 3.4.23.32 extracted from
- Streptomyces pepsin inhibitor-insensitive carboxyl proteinase from Ganoderma lucidum (1984), Agric. Biol. Chem., 48, 1029-1035.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,2-epoxy-3-(4-nitrophenoxy)propane | not | Ganoderma lucidum |  |
| additional information | diazoacetyl-DL-norleucine methyl ester; methyl 2-diazoacetamidohexanoate; not: pepstatin Ac; Streptomyces-pepsin inhibitor (S-PI) | Ganoderma lucidum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
Ganoderma lucidum, gel filtration | Ganoderma lucidum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ganoderma lucidum | - |
similar enzyme | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ganoderma lucidum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture filtrate | - |
Ganoderma lucidum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Ganoderma lucidum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | 15% of the activity with hemoglobin | Ganoderma lucidum | hydrolyzed casein | - |
? | |
| Hemoglobin + H2O | - |
Ganoderma lucidum | Hydrolyzed hemoglobin | - |
? | |
| Oxidized insulin B-chain + H2O | splits preferentially the Phe24-Phe25 bond at the rate 50% for total hydrolysis | Ganoderma lucidum | Hydrolyzed insulin B-chain | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 36000, Ganoderma lucidum, SDS-PAGE | Ganoderma lucidum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 52 | - |
- |
Ganoderma lucidum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
pH 3.2-5.2, 3 h, stable | Ganoderma lucidum |
| 42 | - |
pH 4.0, 30 min, stable below | Ganoderma lucidum |
| 58 | - |
pH 4, 30 min, complete loss of activity | Ganoderma lucidum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 2 | - |
casein | Ganoderma lucidum |
| 3.2 | - |
hemoglobin | Ganoderma lucidum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 5 | 2: about 30% of activity maximum, 5: about 50% of activity maximum, hemoglobin | Ganoderma lucidum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.2 | 5.2 | 37°C, 3 h, stable | Ganoderma lucidum |
| 4 | - |
58°C | Ganoderma lucidum |
| 4 | - |
stable below 42°C, 30 min | Ganoderma lucidum |
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Release 2023.1 (January 2023)
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