Protein Variants | Comment | Organism |
---|---|---|
A213I | increased Km and 50% higher turnover than wild type enzyme | Saccharomyces cerevisiae |
T222A | 50% of wild type turnover | Saccharomyces cerevisiae |
T287A/P288G | 50% of wild type turnover | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
IA3 | highly specific polypeptide inhibitor synthesized by Saccharomyces cerevisiae, a hydrogen bond network also residues K18 and D22 of the inhibitor directly to the catalytic Asp-32 and Tyr-75 residues of the enzyme, thus deadlocking the inhibitor in position, numerous mutants of IA3 created and tested for inhibition, all mutants are found to be inhibitory although to different extent, variations within residues 2-32 at positions other than 18 and 22 appears to have relatively minor effects | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
Lys-Pro-Ile-Glu-Phe-L-nitrophenylalanine-Arg-Leu | T222A mutant, pH 4.7 | Saccharomyces cerevisiae | |
0.12 | - |
Lys-Pro-Ile-Glu-Phe-L-nitrophenylalanine-Arg-Leu | T287A/P288G mutant, pH 4.7 | Saccharomyces cerevisiae | |
0.14 | - |
Lys-Pro-Ile-Glu-Phe-L-nitrophenylalanine-Arg-Leu | wild type enzyme, pH 4.7 | Saccharomyces cerevisiae | |
0.25 | - |
Lys-Pro-Ile-Glu-Phe-L-nitrophenylalanine-Arg-Leu | A213I mutant, pH 4.7 | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
Hydrolysis of proteins with broad specificity for peptide bonds. Cleaves -Leu-Leu-/-Val-Tyr bond in a synthetic substrate. Does not act on esters of Tyr or Arg | Asp-32 and Tyr-75 residues involved in catalysis | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Lys-Pro-Ile-Glu-Phe-L-nitrophenylalanine-Arg-Leu + H2O | - |
Saccharomyces cerevisiae | Lys-Pro-Ile-Glu-Phe + L-nitrophenylalanine-Arg-Leu | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Proteinase A | use of recommended name should be favored | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
21 | - |
Lys-Pro-Ile-Glu-Phe-L-nitrophenylalanine-Arg-Leu | T222A mutant, pH 4.7 | Saccharomyces cerevisiae | |
21 | - |
Lys-Pro-Ile-Glu-Phe-L-nitrophenylalanine-Arg-Leu | T287A/P288G mutant, pH 4.7 | Saccharomyces cerevisiae | |
44 | - |
Lys-Pro-Ile-Glu-Phe-L-nitrophenylalanine-Arg-Leu | wild type enzyme, pH 4.7 | Saccharomyces cerevisiae | |
66 | - |
Lys-Pro-Ile-Glu-Phe-L-nitrophenylalanine-Arg-Leu | A213I mutant, pH 4.7 | Saccharomyces cerevisiae |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
additional information | - |
below 0.1 nM, exact detection impossible | Saccharomyces cerevisiae | IA3 |