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Literature summary for 3.4.23.21 extracted from
- Isolation of a protease from a Rhizopus product (1971), Agric. Biol. Chem., 35, 1668-1675.
No PubMed abstract available
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Rhizopus niveus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM, activates | Rhizopus niveus |  |
| Cu2+ | 1 mM, activates | Rhizopus niveus | |
| Mn2+ | 1 mM, activates | Rhizopus niveus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40300 | - |
Rhizopus niveus, low speed sedimentation without reaching maximum | Rhizopus niveus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhizopus niveus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rhizopus niveus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rhizopus niveus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | - |
Rhizopus niveus | ? | - |
? | |
| Oxidized insulin B-chain + H2O | - |
Rhizopus niveus | Proteolytically cleaved oxidized insulin B-chain | - |
? | |
| Peptides + H2O | obtained from oxidized B-chain of insulin | Rhizopus niveus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Rhizopus niveus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 58 | - |
10 min, 90% loss of activity | Rhizopus niveus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 4 | casein | Rhizopus niveus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2 | - |
0.1 M tartrate buffer, 24 h, loses most of its activity, retains its high activity if incubated in 0.1 M tartrate buffer of pH 3.0-4.0 or in 1.0 M acetate buffer of pH 4.5-5.5 | Rhizopus niveus |
| 6.5 | 7.5 | 0.1 M phosphate buffer, 24 h, loses most of its activity, retains its high activity if incubated in 0.1 M tartrate buffer of pH 3.0-4.0 or in 1.0 M acetate buffer of 4.5-5.5 | Rhizopus niveus |
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Release 2023.1 (January 2023)
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