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Literature summary for 3.4.23.20 extracted from

  • Cao, Q.N.; Stubbs, M.; Ngo, K.Q.; Ward, M.; Cunningham, A.; Pai, E.F.; Tu, G.C.; Hofmann, T.
    Penicillopepsin-JT2, a recombinant enzyme from Penicillium janthinellum and the contribution of a hydrogen bond in subsite S3 to k(cat) (2000), Protein Sci., 9, 991-1001.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene pepA, nucleotide sequence determined, inserted into expression vector pGPT-pyrG1, expressed in an aspartic proteinase-free strain of Aspergillus niger var. awamori Penicillium janthinellum

Protein Variants

Protein Variants Comment Organism
T219A constructed by cassette insertion Penicillium janthinellum
T219G constructed by cassette insertion Penicillium janthinellum
T219S constructed by cassette insertion Penicillium janthinellum
T219V constructed by cassette insertion Penicillium janthinellum

Inhibitors

Inhibitors Comment Organism Structure
pepstatin
-
Penicillium janthinellum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.21
-
Ac-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, wild-type Pencillopepsin JT-2 Penicillium janthinellum
0.3
-
Ac-Ala-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219S Penicillium janthinellum
0.32
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, wild-type Pencillopepsin JT-2 Penicillium janthinellum
0.35
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, wild-type Pencillopepsin JT-2 Penicillium janthinellum
0.37
-
Ac-Ala-Ala-Lys-(NO2)Phe-amide pH 5.0, 25°C, and mutant T219S Penicillium janthinellum
0.39
-
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T291S Penicillium janthinellum
0.4
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219G Penicillium janthinellum
0.4
-
Ac-Lys-(NO2)Phe-amide pH 5.0, 25°C, wild-type Pencillopepsin JT-2 Penicillium janthinellum
0.4
-
Ac-Ala-Lys-(NO2)Phe-amide pH 5.0, 25°C, wild-type Pencillopepsin JT-2 Penicillium janthinellum
0.41
-
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, wild-type Pencillopepsin JT-2 Penicillium janthinellum
0.42
-
Ac-Ala-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219S Penicillium janthinellum
0.45
-
Ac-Ala-Ala-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219G Penicillium janthinellum
0.45
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219G Penicillium janthinellum
0.45
-
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219V Penicillium janthinellum
0.46
-
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219S Penicillium janthinellum
0.46
-
Ac-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219V Penicillium janthinellum
0.46
-
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, wild-type Pencillopepsin JT-2 Penicillium janthinellum
0.48
-
Ac-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219G Penicillium janthinellum
0.48
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219S Penicillium janthinellum
0.48
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219S Penicillium janthinellum
0.5
-
Ac-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219S Penicillium janthinellum
0.5
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219V Penicillium janthinellum
0.5
-
Ac-Ala-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, wild-type Pencillopepsin JT-2 Penicillium janthinellum
0.5
-
Ac-Ala-Ala-Lys-(NO2)Phe-amide pH 5.0, 25°C, wild-type Pencillopepsin JT-2 Penicillium janthinellum
0.51
-
Ac-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219S Penicillium janthinellum
0.54
-
Ac-Ala-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219G Penicillium janthinellum
0.55
-
Ac-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219V Penicillium janthinellum
0.55
-
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T291G Penicillium janthinellum
0.57
-
Ac-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219V Penicillium janthinellum
0.59
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219A Penicillium janthinellum
0.59
-
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T291V Penicillium janthinellum
0.59
-
Ac-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, wild-type Pencillopepsin JT-2 Penicillium janthinellum
0.6
-
Ac-Ala-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219A Penicillium janthinellum
0.6
-
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219A Penicillium janthinellum
0.6
-
Ac-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219G and T219S Penicillium janthinellum
0.6
-
Ac-Ala-Ala-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219V Penicillium janthinellum
0.6
-
Ac-Ala-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219V Penicillium janthinellum
0.65
-
Ac-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219A Penicillium janthinellum
0.68
-
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T291A Penicillium janthinellum
0.71
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219V and T219A Penicillium janthinellum
0.74
-
Ac-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219G Penicillium janthinellum
0.75
-
Ac-Ala-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219A Penicillium janthinellum
0.77
-
Ac-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219A Penicillium janthinellum
0.8
-
Ac-Ala-Ala-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219A Penicillium janthinellum
0.8
-
Ac-Ala-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219V Penicillium janthinellum
0.83
-
Ac-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219A Penicillium janthinellum
0.83
-
Ac-Ala-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219G Penicillium janthinellum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33700 34000 mass spectrometry Penicillium janthinellum

Organism

Organism UniProt Comment Textmining
Penicillium janthinellum
-
-
-
Penicillium janthinellum NRRL 905
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein PencillopepsinJT2, glycosylated form of the active enzyme Penicillium janthinellum

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Penicillium janthinellum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide + H2O
-
Penicillium janthinellum ?
-
?
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide + H2O
-
Penicillium janthinellum ?
-
?
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide + H2O
-
Penicillium janthinellum ?
-
?
Ac-Ala-Ala-Lys-(NO2)Phe-amide + H2O
-
Penicillium janthinellum ?
-
?
Ac-Ala-Ala-Lys-(NO2)Phe-amide + H2O
-
Penicillium janthinellum NRRL 905 ?
-
?
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide + H2O
-
Penicillium janthinellum ?
-
?
Ac-Ala-Lys-(NO2)Phe-Ala-amide + H2O
-
Penicillium janthinellum ?
-
?
Ac-Ala-Lys-(NO2)Phe-Ala-amide + H2O
-
Penicillium janthinellum NRRL 905 ?
-
?
Ac-Ala-Lys-(NO2)Phe-amide + H2O
-
Penicillium janthinellum ?
-
?
Ac-Ala-Lys-(NO2)Phe-amide + H2O
-
Penicillium janthinellum NRRL 905 ?
-
?
Ac-Ala-Lys-Ala-(NO2)Phe-amide + H2O
-
Penicillium janthinellum ?
-
?
Ac-Lys-(NO2)Phe- Ala-amide + H2O
-
Penicillium janthinellum ?
-
?
Ac-Lys-(NO2)Phe- Ala-amide + H2O
-
Penicillium janthinellum NRRL 905 ?
-
?
Ac-Lys-(NO2)Phe-Ala-Ala-amide + H2O
-
Penicillium janthinellum ?
-
?
Ac-Lys-(NO2)Phe-amide + H2O
-
Penicillium janthinellum ?
-
?
Ac-Lys-(NO2)Phe-amide + H2O
-
Penicillium janthinellum NRRL 905 ?
-
?

Synonyms

Synonyms Comment Organism
pencillopepsin-JT1 native enzyme Penicillium janthinellum
pencillopepsin-JT2 recombinant enzyme Penicillium janthinellum
Penicillium janthinellum aspartic proteinase
-
Penicillium janthinellum
Peptidase A
-
Penicillium janthinellum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0016
-
Ac-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219V Penicillium janthinellum
0.0019
-
Ac-Ala-Ala-Lys-(NO2)Phe-amide pH 5.0, 25°C, and mutant T219V Penicillium janthinellum
0.002
-
Ac-Lys-(NO2)Phe-amide pH 5.0, 25°C, wild-type pencillopepsin JT-2 and mutant T219A Penicillium janthinellum
0.003
-
Ac-Ala-Ala-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219G Penicillium janthinellum
0.003
-
Ac-Ala-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219G Penicillium janthinellum
0.003
-
Ac-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219S Penicillium janthinellum
0.003
-
Ac-Ala-Lys-Ala-(NO2)Phe-amide pH 5.0, 25°C, mutant T219S Penicillium janthinellum
0.003
-
Ac-Ala-Lys-(NO2)Phe-amide pH 5.0, 25°C, wild-type pencillopepsin JT-2 Penicillium janthinellum
0.004
-
Ac-Ala-Ala-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219A Penicillium janthinellum
0.005
-
Ac-Ala-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219A Penicillium janthinellum
0.005
-
Ac-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219G Penicillium janthinellum
0.0054
-
Ac-Ala-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219V Penicillium janthinellum
0.042
-
Ac-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219A Penicillium janthinellum
0.046
-
Ac-Ala-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219A Penicillium janthinellum
0.05
-
Ac-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219V Penicillium janthinellum
0.07
-
Ac-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219S Penicillium janthinellum
0.09
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219A Penicillium janthinellum
0.09
-
Ac-Ala-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219S Penicillium janthinellum
0.11
-
Ac-Ala-Ala-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219S Penicillium janthinellum
0.12
-
Ac-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219A Penicillium janthinellum
0.12
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219V Penicillium janthinellum
0.13
-
Ac-Ala-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219G Penicillium janthinellum
0.14
-
Ac-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219G Penicillium janthinellum
0.15
-
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219S Penicillium janthinellum
0.17
-
Ac-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219V Penicillium janthinellum
0.23
-
Ac-Ala-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219V Penicillium janthinellum
0.24
-
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219G Penicillium janthinellum
0.29
-
Ac-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, wild-type pencillopepsin JT-2 Penicillium janthinellum
0.3
-
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219A Penicillium janthinellum
0.3
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219V Penicillium janthinellum
0.3
-
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, wild-type pencillopepsin JT-2 Penicillium janthinellum
0.33
-
Ac-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219S Penicillium janthinellum
0.35
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219G Penicillium janthinellum
0.35
-
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T291V Penicillium janthinellum
0.36
-
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T291A Penicillium janthinellum
0.36
-
Ac-Ala-Ala-Lys-(NO2)Phe-amide pH 5.0, 25°C, wild-type pencillopepsin JT-2 Penicillium janthinellum
0.4
-
Ac-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, wild-type pencillopepsin JT-2 Penicillium janthinellum
0.43
-
Ac-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219G Penicillium janthinellum
0.45
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219A Penicillium janthinellum
0.45
-
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219V and T219G Penicillium janthinellum
0.45
-
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T291G Penicillium janthinellum
0.5
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219G Penicillium janthinellum
0.6
-
Ac-Ala-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, wild-type pencillopepsin JT-2 Penicillium janthinellum
3.8
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219S Penicillium janthinellum
14.9
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219S Penicillium janthinellum
15
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, wild-type pencillopepsin JT-2 Penicillium janthinellum
16.1
-
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T291S Penicillium janthinellum
34
-
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, wild-type pencillopepsin JT-2 Penicillium janthinellum
40
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, wild-type pencillopepsin JT-2 Penicillium janthinellum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00000001
-
pepstatin pH 5.0, 37°C, wild-type Pencillopepsin JT-2 Penicillium janthinellum
0.0000004
-
pepstatin pH 5.0, 37°C, mutant T219S Penicillium janthinellum
0.00055
-
pepstatin pH 5.0, 37°C, mutant T219A Penicillium janthinellum
0.0016
-
pepstatin pH 5.0, 37°C, mutant T219V Penicillium janthinellum
0.0018
-
pepstatin pH 5.0, 37°C, mutant T219G Penicillium janthinellum