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Literature summary for 3.4.23.2 extracted from
- Roles of Tyr13 and Phe219 in the unique substrate specificity of pepsin B (2006), Biochemistry, 45, 14415-14426.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Saccharomyces cerevisiae | Canis lupus familiaris |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F219S | mutant exhibits broad activity against various peptides, with retention of high activity against peptides with Gly at P2 | Canis lupus familiaris |
| Y13A | mutant shows new hydrolytic activity against several peptides, whereas the activity against peptides with Gly at P2 is decreased significantly. The newly obtained activity of the Tyr13Ala mutant is low | Canis lupus familiaris |
| Y13A/F219S | mutant demonstrats general proteolytic activity, hydrolyzing various types of peptides very efficiently, although activity against peptides with Gly at P2 is decreased. The activity against typical peptides, such as KPAEFARL, KPAEFLRL, and KPGEFARL, is increased about 40times by the double mutation. The extent being much higher than that with single mutations | Canis lupus familiaris |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.35 | - |
KPAEFFRL | mutant enzyme Y13A | Canis lupus familiaris |  |
| 0.42 | - |
KPAEFFRL | mutant enzyme F2119S | Canis lupus familiaris | |
| 0.5 | - |
KPAAFFRL | mutant enzyme F2119S | Canis lupus familiaris | |
| 0.67 | - |
KPAAFFRL | mutant enzyme Y13A/F219S | Canis lupus familiaris | |
| 0.71 | - |
KPAEFFRL | mutant enzyme Y13A/F219S | Canis lupus familiaris | |
| 0.95 | - |
KPAEFARL | wild-type enzyme | Canis lupus familiaris | |
| 1.3 | - |
KPAEFARL | mutant enzyme Y13A/F219S | Canis lupus familiaris | |
| 2.2 | - |
KPGGFARL | wild-type enzyme | Canis lupus familiaris | |
| 2.2 | - |
KPGGFARL | mutant enzyme F2119S | Canis lupus familiaris | |
| 2.5 | - |
KPAGFARL | mutant enzyme F2119S | Canis lupus familiaris | |
| 4 | - |
KPAGFARL | wild-type enzyme | Canis lupus familiaris | |
| 4 | - |
KPAEFLRL | mutant enzyme Y13A/F219S | Canis lupus familiaris | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Canis lupus familiaris | Q2AB25 | fragment | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Canis lupus familiaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Gelatin + H2O | - |
Canis lupus familiaris | ? | - |
? | |
| Hemoglobin + H2O | - |
Canis lupus familiaris | ? | - |
? | |
| KPAAFFRL + H2O | - |
Canis lupus familiaris | KPAAF + FRL | - |
? | |
| KPAEFARL + H2O | - |
Canis lupus familiaris | KPAEF + ARL | - |
? | |
| KPAEFFRL + H2O | - |
Canis lupus familiaris | KPAEF + FRL | - |
? | |
| KPAEFLRL + H2O | - |
Canis lupus familiaris | KPAEF + LRL | - |
? | |
| KPAGFARL + H2O | - |
Canis lupus familiaris | KPAGF + ARL | - |
? | |
| KPAGFFRL + H2O | - |
Canis lupus familiaris | KPAGF + FRL | - |
? | |
| KPAGFGRL + H2O | - |
Canis lupus familiaris | KPAGF + GRL | - |
? | |
| KPAGFLRL + H2O | - |
Canis lupus familiaris | KPAGF + LRL | - |
? | |
| KPAGFVRL + H2O | - |
Canis lupus familiaris | KPAGF + VRL | - |
? | |
| KPAKFLRL + H2O | - |
Canis lupus familiaris | KPAKF + LRL | - |
? | |
| KPEEFFRL + H2O | - |
Canis lupus familiaris | KPEEF + FRL | - |
? | |
| KPEGFLRL + H2O | - |
Canis lupus familiaris | KPEGF + LRL | - |
? | |
| KPGEFARL + H2O | - |
Canis lupus familiaris | KPGEF + ARL | - |
? | |
| KPGGF + LRL | - |
Canis lupus familiaris | KPGGF + LRL | - |
? | |
| KPGGFARL + H2O | - |
Canis lupus familiaris | KPGGF + ARL | - |
? | |
| KPIGFLRL + H2O | - |
Canis lupus familiaris | KPIGF + LRL | - |
? | |
| KPKEFFRL + H2O | - |
Canis lupus familiaris | KPKEF + FRL | - |
? | |
| KPKGFLRL + H2O | - |
Canis lupus familiaris | KPKGF + LRL | - |
? | |
| KPTGFLRL + H2O | - |
Canis lupus familiaris | KPTGF + LRL | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
KPAEFFRL | mutant enzyme F2119S | Canis lupus familiaris | |
| 1.2 | - |
KPAAFFRL | mutant enzyme F2119S | Canis lupus familiaris | |
| 2.5 | - |
KPAEFFRL | mutant enzyme Y13A | Canis lupus familiaris | |
| 3.4 | - |
KPAEFARL | wild-type enzyme | Canis lupus familiaris | |
| 11 | - |
KPAAFFRL | mutant enzyme Y13A/F219S | Canis lupus familiaris | |
| 18 | - |
KPAEFFRL | mutant enzyme Y13A/F219S | Canis lupus familiaris | |
| 91 | - |
KPAEFARL | mutant enzyme Y13A/F219S | Canis lupus familiaris | |
| 113 | - |
KPAEFLRL | mutant enzyme Y13A/F219S | Canis lupus familiaris | |
| 116 | - |
KPGGFARL | mutant enzyme F2119S | Canis lupus familiaris | |
| 130 | - |
KPAGFARL | mutant enzyme F2119S | Canis lupus familiaris | |
| 134 | - |
KPGGFARL | wild-type enzyme | Canis lupus familiaris | |
| 169 | - |
KPAGFARL | wild-type enzyme | Canis lupus familiaris | |
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