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Literature summary for 3.4.23.18 extracted from
- Comparative specificity of microbial acid proteinases for synthetic peptides. 3. Relationship with their trypsinogen activating ability (1973), Arch. Biochem. Biophys., 157, 561-572.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.4 | - |
Benzyloxycarbonyl-Ala-Lys-Ala-Ala-Ala | pH 3.2, Aspergillus niger | Aspergillus niger |  |
| 3.5 | - |
Benzyloxycarbonyl-Ala-Ala-Lys-Ala-Ala-Ala | pH 3.2, Aspergillus niger | Aspergillus niger | |
| 4.1 | 4.3 | Benzyloxycarbonyl-Lys-Leu-Ala-Ala | Aspergillus niger | Aspergillus niger | |
| 4.5 | - |
Benzyloxycarbonyl-Ala-Ala-Lys-Ala-Ala-Ala | pH 4.5, Aspergillus niger | Aspergillus niger | |
| 8.2 | - |
Benzyloxycarbonyl-Lys-Ala-Ala-Ala | pH 3.2, Aspergillus niger | Aspergillus niger | |
| 10.6 | - |
Benzyloxycarbonyl-Lys-Ala-Ala-Ala | pH 4.5, Aspergillus niger | Aspergillus niger | |
| 10.9 | - |
Benzyloxycarbonyl-Ala-Lys-Ala-Ala-Ala | pH 4.5, Aspergillus niger | Aspergillus niger | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
| Aspergillus niger B | - |
- |
- |
| Aspergillus phoenicis | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Benzyloxycarbonyl-Ala-Ala-Lys-Ala-Ala-Ala + H2O | best substrate | Aspergillus niger | Benzyloxycarbonyl-Ala-Ala-Lys + Ala-Ala-Ala | - |
? | |
| Benzyloxycarbonyl-Ala-Lys-Ala-Ala-Ala + H2O | - |
Aspergillus niger | Benzyloxycarbonyl-Ala-Lys + Ala-Ala-Ala | - |
? | |
| Benzyloxycarbonyl-Lys-Ala-Ala + H2O | poor substrate | Aspergillus niger | Benzyloxycarbonyl-Lys + Ala-Ala | - |
? | |
| Benzyloxycarbonyl-Lys-Ala-Ala + H2O | poor substrate | Aspergillus phoenicis | Benzyloxycarbonyl-Lys + Ala-Ala | - |
? | |
| Benzyloxycarbonyl-Lys-Ala-Ala-Ala + H2O | - |
Aspergillus niger | Benzyloxycarbonyl-Lys + Ala-Ala-Ala | - |
? | |
| Benzyloxycarbonyl-Lys-Ala-Ala-Ala-Ala + H2O | poor substrate | Aspergillus niger | Benzyloxycarbonyl-Lys + Ala-Ala-Ala-Ala | - |
? | |
| Benzyloxycarbonyl-Lys-Leu-Ala + H2O | - |
Aspergillus niger | Benzyloxycarbonyl-Lys + Leu-Ala | - |
? | |
| Benzyloxycarbonyl-Lys-Leu-Ala + H2O | - |
Aspergillus phoenicis | Benzyloxycarbonyl-Lys + Leu-Ala | - |
? | |
| Benzyloxycarbonyl-Lys-Leu-Ala + H2O | - |
Aspergillus niger B | Benzyloxycarbonyl-Lys + Leu-Ala | - |
? | |
| Benzyloxycarbonyl-Lys-Leu-Ala-Ala + H2O | - |
Aspergillus niger | Benzyloxycarbonyl-Lys + Leu-Ala-Ala | - |
? | |
| Benzyloxycarbonyl-Lys-Phe-Ala + H2O | - |
Aspergillus niger | Benzyloxycarbonyl-Lys + Phe-Ala | - |
? | |
| Benzyloxycarbonyl-Lys-Phe-Ala + H2O | - |
Aspergillus phoenicis | Benzyloxycarbonyl-Lys + Phe-Ala | - |
? | |
| Benzyloxycarbonyl-Lys-Phe-Ala + H2O | - |
Aspergillus niger B | Benzyloxycarbonyl-Lys + Phe-Ala | - |
? | |
| additional information | specificity | Aspergillus niger | ? | - |
? | |
| additional information | specificity | Aspergillus phoenicis | ? | - |
? | |
| additional information | no hydrolysis of benzyloxycarbonyl-Lys-Gly-Ala, benzyloxycarbonyl-Lys-(D)-Leu-Ala | Aspergillus niger | ? | - |
? | |
| additional information | no hydrolysis of benzyloxycarbonyl-Lys-Gly-Ala, benzyloxycarbonyl-Lys-(D)-Leu-Ala | Aspergillus phoenicis | ? | - |
? | |
| additional information | specificity | Aspergillus niger B | ? | - |
? | |
| additional information | no hydrolysis of benzyloxycarbonyl-Lys-Gly-Ala, benzyloxycarbonyl-Lys-(D)-Leu-Ala | Aspergillus niger B | ? | - |
? | |
| Trypsinogen + H2O | - |
Aspergillus niger | ? | - |
? | |
| Trypsinogen + H2O | - |
Aspergillus phoenicis | ? | - |
? | |
| Trypsinogen + H2O | - |
Aspergillus niger B | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
assay at | Aspergillus niger |
| 40 | - |
assay at | Aspergillus phoenicis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.044 | - |
Benzyloxycarbonyl-Lys-Ala-Ala-Ala | pH 3.2, Aspergillus niger | Aspergillus niger | |
| 0.17 | - |
Benzyloxycarbonyl-Lys-Ala-Ala-Ala | pH 4.5, Aspergillus niger | Aspergillus niger | |
| 0.38 | - |
Benzyloxycarbonyl-Lys-Leu-Ala-Ala | pH 3.2, Aspergillus niger | Aspergillus niger | |
| 0.46 | - |
Benzyloxycarbonyl-Ala-Lys-Ala-Ala-Ala | pH 3.2, Aspergillus niger | Aspergillus niger | |
| 0.67 | - |
Benzyloxycarbonyl-Lys-Leu-Ala-Ala | pH 4.5, Aspergillus niger | Aspergillus niger | |
| 2.18 | - |
Benzyloxycarbonyl-Ala-Lys-Ala-Ala-Ala | pH 4.5, Aspergillus niger | Aspergillus niger | |
| 9.32 | - |
Benzyloxycarbonyl-Ala-Ala-Lys-Ala-Ala-Ala | pH 3.2, Aspergillus niger | Aspergillus niger | |
| 30.5 | - |
Benzyloxycarbonyl-Ala-Ala-Lys-Ala-Ala-Ala | pH 4.5, Aspergillus niger | Aspergillus niger | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 5 | synthetic peptides | Aspergillus niger |
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Release 2023.1 (January 2023)
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