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Literature summary for 3.4.23.16 extracted from
- Highly conserved glycine 86 and arginine 87 residues contribute differently to the structure and activity of the mature HIV-1 protease (2010), Proteins, 78, 1015-1025.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| mutant enzymes G86A and G86S are expressed in Escherichia coli BL21(DE3) cells | Human immunodeficiency virus 1 |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| mutant enzymes G86A and G86S in complex with darunavir or DMP323, hanging drop vapor diffusion method, using 10% (w/v) sodium chloride and 0.1 M MES buffer at pH 6.5 and 4°C | Human immunodeficiency virus 1 |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| G86A | the mutant exhibits about 6000fold lower catalytic activity than the wild type enzyme | Human immunodeficiency virus 1 |
| G86S | the mutant has undetectable catalytic activity | Human immunodeficiency virus 1 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| darunavir | - |
Human immunodeficiency virus 1 |  |
| DMP323 | - |
Human immunodeficiency virus 1 | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Gag polyprotein + H2O | Human immunodeficiency virus 1 | - |
? | - |
? | |
| Gag-Pol polyprotein + H2O | Human immunodeficiency virus 1 | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Human immunodeficiency virus 1 | P03367 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Human immunodeficiency virus 1 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Gag polyprotein + H2O | - |
Human immunodeficiency virus 1 | ? | - |
? | |
| Gag-Pol polyprotein + H2O | - |
Human immunodeficiency virus 1 | ? | - |
? | |
| Lys-Ala-Arg-Val-Nle-(4-nitrophenylalanine)-Glu-Ala-Nle-NH2 + H2O | - |
Human immunodeficiency virus 1 | Lys-Ala-Arg-Val-Nle + (4-nitrophenylalanine)-Glu-Ala-Nle-NH2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HIV-1 PR | - |
Human immunodeficiency virus 1 |
| HIV-1 protease | - |
Human immunodeficiency virus 1 |
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Release 2023.1 (January 2023)
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