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Literature summary for 3.4.23.15 extracted from

  • Bukhtiyarov, Y.; Zecher, M.; Panemangalore, R.; Wu, Z.; Bruno, J.G.; Yuan, J.; Xu, Z.; Dillard, L.W.; McGeehan, G.M.; Harrison, R.K.; Scott, B.B.
    Cloning, characterization and site-directed mutagenesis of canine renin (2007), J. Biochem., 142, 671-680.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
full-length prorenin is expressed in HEK 293 cells Canis lupus familiaris

Protein Variants

Protein Variants Comment Organism
G149S/S150T mutant enzyme shows lower specific activities than native renin. 50% loss in specific activity with 4-(4'-(dimethylaminophenylazo))-benzoyl-gamma-aminobutyryl-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-EDANS and 4-(4'-(dimethylaminophenylazo))-benzoyl-gamma-aminobutyryl-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-EDANS. Mutation has no effect on substrate specificity of canine renin Canis lupus familiaris
G149S/S150T/V286L mutant enzyme shows lower specific activities than native renin Canis lupus familiaris
V286L mutant enzyme shows lower specific activities than native renin Canis lupus familiaris

Inhibitors

Inhibitors Comment Organism Structure
aliskiren
-
Canis lupus familiaris
pepstatin A
-
Canis lupus familiaris
SR 42128
-
Canis lupus familiaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
acetyl-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu-Val-Tyr-Ser
-
Canis lupus familiaris

Organism

Organism UniProt Comment Textmining
Canis lupus familiaris Q6DYE7
-
-

Purification (Commentary)

Purification (Comment) Organism
full-length prorenin is expressed in HEK 293 cells, purified and converted to its active form by trypsin-mediated cleavage of the 43 residue propeptide Canis lupus familiaris

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Canis lupus familiaris
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-(4'-(dimethylaminophenylazo))-benzoyl-gamma-aminobutyryl-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-EDANS + H2O
-
Canis lupus familiaris 4-(4'-(dimethylaminophenylazo))-benzoyl-gamma-aminobutyryl-Ile-His-Pro-Phe-His-Leu + Val-Ile-His-Thr-EDANS
-
?
acetyl-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu-Val-Tyr-Ser + H2O
-
Canis lupus familiaris acetyl-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu + Leu-Val-Tyr-Ser
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Canis lupus familiaris

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0000006
-
-
Canis lupus familiaris aliskiren
0.0000023
-
-
Canis lupus familiaris SR 42128
0.0008
-
-
Canis lupus familiaris pepstatin A