Cloned (Comment) | Organism |
---|---|
full-length prorenin is expressed in HEK 293 cells | Canis lupus familiaris |
Protein Variants | Comment | Organism |
---|---|---|
G149S/S150T | mutant enzyme shows lower specific activities than native renin. 50% loss in specific activity with 4-(4'-(dimethylaminophenylazo))-benzoyl-gamma-aminobutyryl-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-EDANS and 4-(4'-(dimethylaminophenylazo))-benzoyl-gamma-aminobutyryl-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-EDANS. Mutation has no effect on substrate specificity of canine renin | Canis lupus familiaris |
G149S/S150T/V286L | mutant enzyme shows lower specific activities than native renin | Canis lupus familiaris |
V286L | mutant enzyme shows lower specific activities than native renin | Canis lupus familiaris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
aliskiren | - |
Canis lupus familiaris | |
pepstatin A | - |
Canis lupus familiaris | |
SR 42128 | - |
Canis lupus familiaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
acetyl-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu-Val-Tyr-Ser | - |
Canis lupus familiaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Canis lupus familiaris | Q6DYE7 | - |
- |
Purification (Comment) | Organism |
---|---|
full-length prorenin is expressed in HEK 293 cells, purified and converted to its active form by trypsin-mediated cleavage of the 43 residue propeptide | Canis lupus familiaris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Canis lupus familiaris | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-(4'-(dimethylaminophenylazo))-benzoyl-gamma-aminobutyryl-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-EDANS + H2O | - |
Canis lupus familiaris | 4-(4'-(dimethylaminophenylazo))-benzoyl-gamma-aminobutyryl-Ile-His-Pro-Phe-His-Leu + Val-Ile-His-Thr-EDANS | - |
? | |
acetyl-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu-Val-Tyr-Ser + H2O | - |
Canis lupus familiaris | acetyl-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu + Leu-Val-Tyr-Ser | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Canis lupus familiaris |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0000006 | - |
- |
Canis lupus familiaris | aliskiren | |
0.0000023 | - |
- |
Canis lupus familiaris | SR 42128 | |
0.0008 | - |
- |
Canis lupus familiaris | pepstatin A |