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Literature summary for 3.4.23.15 extracted from

  • Nabi, A.H.; Kageshima, A.; Uddin, M.N.; Nakagawa, T.; Park, E.Y.; Suzuki, F.
    Binding properties of rat prorenin and renin to the recombinant rat renin/prorenin receptor prepared by a baculovirus expression system (2006), Int. J. Mol. Med., 18, 483-488.
    View publication on PubMed
Search for more literature for 3.4.23.15

Cloned(Commentary)

Cloned (Comment) Organism
expression of prorenin in CHO cells, expression of the FLAG-tagged renin/prorenin receptor in the cytoplasm of Trichoplusia ni Tn-5B1-4 insect cells using the baculovirus transfection system, subcloning in Escherichia coli strain DH10 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information binding affinities of recombinant prorenin and renin to the recombinant renin/prorenin receptor, kinetics, overview Rattus norvegicus
0.0033
-
 angiotensinogen recombinant, immobilized renin and prorenin Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 x * 46000, recombinant prorenin, SDS-PAGE, x * 42000, recombinant renin, SDS-PAGE Rattus norvegicus
46000
-
 x * 46000, recombinant prorenin, SDS-PAGE, x * 42000, recombinant renin, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
angiotensinogen + H2O Rattus norvegicus
-
 angiotensin I + angiotensinogen propeptide
-
 ?
additional information Rattus norvegicus possible binding mechanism of prorenin and renin to the renin/prorenin receptor, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the inactive prorenin needs to be proteolytically activated to the mature active renin, activation of the recombinant prorenin in vitro by trypsin at 25°C and pH 6.5 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
angiotensinogen + H2O
-
 Rattus norvegicus angiotensin I + angiotensinogen propeptide
-
 ?
additional information possible binding mechanism of prorenin and renin to the renin/prorenin receptor, overview Rattus norvegicus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 46000, recombinant prorenin, SDS-PAGE, x * 42000, recombinant renin, SDS-PAGE Rattus norvegicus