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Literature summary for 3.4.23.12 extracted from

  • Athauda, S.B.; Matsumoto, K.; Rajapakshe, S.; Kuribayashi, M.; Kojima, M.; Kubomura-Yoshida, N.; Iwamatsu, A.; Shibata, C.; Inoue, H.; Takahashi, K.
    Enzymic and structural characterization of nepenthesin, a unique member of a novel subfamily of aspartic proteinases (2004), Biochem. J., 381, 295-306.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
nepenthesin I and II Nepenthes distillatoria

Inhibitors

Inhibitors Comment Organism Structure
cupric ions strong inhibition of nepenthesin I and II by diazoacetyl-DL-norleucine in presence of cupric ions, no inactivation in absence of cupric ions Nepenthes distillatoria
diazoacetyl-DL-norleucine strong inhibition of nepenthesin I and II in presence of cupric ions, no inactivation in absence of cupric ions Nepenthes distillatoria
pepstatin A 0.1 mM, complete inhibition of nepenthesin I and II Nepenthes distillatoria

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
1 * 35000, nepenthesin II, SDS-PSGE under non-reducing conditions Nepenthes distillatoria
37476
-
1 * 37476, nepenthesin Ia, calculation from nucleotide sequence Nepenthes distillatoria
37511
-
1 * 37511, nepenthesin II, calculation from nucleotide sequence Nepenthes distillatoria
37519
-
1 * 37519, nepenthesin Ib, calculation from nucleotide sequence Nepenthes distillatoria
45000
-
nepenthesin II, gel filtration Nepenthes distillatoria
51000
-
nepenthesin I, gel filtration Nepenthes distillatoria

Organism

Organism UniProt Comment Textmining
Nepenthes distillatoria
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein nepenthesin I contains 6 potential N-glycosylation sites, nepenthesin II has none Nepenthes distillatoria

Purification (Commentary)

Purification (Comment) Organism
nepenthesin I and II Nepenthes distillatoria

Source Tissue

Source Tissue Comment Organism Textmining
pitcher secretion
-
Nepenthes distillatoria
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Nepenthes distillatoria

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acid-denatured haemoglobin + H2O
-
Nepenthes distillatoria ?
-
?
oxidized insulin B chain + H2O nepenthesin I: 80% cleavage of Phe24-Phe25, 67% cleavage of Glu13-Ala14, 50% cleavage of Leu6-cysteic-acid7, 38% cleavage of Leu15-Tyr16 and 33% cleavage of Tyr16-Leu17 Nepenthes distillatoria ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 35000, nepenthesin II, SDS-PSGE under non-reducing conditions Nepenthes distillatoria
monomer 1 * 37476, nepenthesin Ia, calculation from nucleotide sequence Nepenthes distillatoria
monomer 1 * 37511, nepenthesin II, calculation from nucleotide sequence Nepenthes distillatoria
monomer 1 * 37519, nepenthesin Ib, calculation from nucleotide sequence Nepenthes distillatoria
monomer 1 * 45000, nepenthesin I, SDS-PSGE under non-reducing conditions Nepenthes distillatoria

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
nepenthesin II Nepenthes distillatoria
55
-
nepenthesin I Nepenthes distillatoria

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
-
Nepenthes distillatoria

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
pH 3.0, nepenthesin I loses 4% of its activity after 7 days, nepenthesin II loses 10% of its activity after 7 d Nepenthes distillatoria
50
-
pH 3.0, nepenthesin I loses 40% of its activity after 30 d, nepenthesin II loses 56% of its activity after 30 d Nepenthes distillatoria

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6
-
reaction with acid-denatured haemoglobin, nepenthesin I and II Nepenthes distillatoria

pI Value

Organism Comment pI Value Maximum pI Value
Nepenthes distillatoria nepenthesin II, calculation from nucleotide sequence
-
3.09
Nepenthes distillatoria nepenthesin Ia and Ib, calculation from nucleotide sequence
-
3.94