Cloned (Comment) | Organism |
---|---|
nepenthesin I and II | Nepenthes distillatoria |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cupric ions | strong inhibition of nepenthesin I and II by diazoacetyl-DL-norleucine in presence of cupric ions, no inactivation in absence of cupric ions | Nepenthes distillatoria | |
diazoacetyl-DL-norleucine | strong inhibition of nepenthesin I and II in presence of cupric ions, no inactivation in absence of cupric ions | Nepenthes distillatoria | |
pepstatin A | 0.1 mM, complete inhibition of nepenthesin I and II | Nepenthes distillatoria |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
1 * 35000, nepenthesin II, SDS-PSGE under non-reducing conditions | Nepenthes distillatoria |
37476 | - |
1 * 37476, nepenthesin Ia, calculation from nucleotide sequence | Nepenthes distillatoria |
37511 | - |
1 * 37511, nepenthesin II, calculation from nucleotide sequence | Nepenthes distillatoria |
37519 | - |
1 * 37519, nepenthesin Ib, calculation from nucleotide sequence | Nepenthes distillatoria |
45000 | - |
nepenthesin II, gel filtration | Nepenthes distillatoria |
51000 | - |
nepenthesin I, gel filtration | Nepenthes distillatoria |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nepenthes distillatoria | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | nepenthesin I contains 6 potential N-glycosylation sites, nepenthesin II has none | Nepenthes distillatoria |
Purification (Comment) | Organism |
---|---|
nepenthesin I and II | Nepenthes distillatoria |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pitcher secretion | - |
Nepenthes distillatoria | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Nepenthes distillatoria |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acid-denatured haemoglobin + H2O | - |
Nepenthes distillatoria | ? | - |
? | |
oxidized insulin B chain + H2O | nepenthesin I: 80% cleavage of Phe24-Phe25, 67% cleavage of Glu13-Ala14, 50% cleavage of Leu6-cysteic-acid7, 38% cleavage of Leu15-Tyr16 and 33% cleavage of Tyr16-Leu17 | Nepenthes distillatoria | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 35000, nepenthesin II, SDS-PSGE under non-reducing conditions | Nepenthes distillatoria |
monomer | 1 * 37476, nepenthesin Ia, calculation from nucleotide sequence | Nepenthes distillatoria |
monomer | 1 * 37511, nepenthesin II, calculation from nucleotide sequence | Nepenthes distillatoria |
monomer | 1 * 37519, nepenthesin Ib, calculation from nucleotide sequence | Nepenthes distillatoria |
monomer | 1 * 45000, nepenthesin I, SDS-PSGE under non-reducing conditions | Nepenthes distillatoria |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
nepenthesin II | Nepenthes distillatoria |
55 | - |
nepenthesin I | Nepenthes distillatoria |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Nepenthes distillatoria |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
pH 3.0, nepenthesin I loses 4% of its activity after 7 days, nepenthesin II loses 10% of its activity after 7 d | Nepenthes distillatoria |
50 | - |
pH 3.0, nepenthesin I loses 40% of its activity after 30 d, nepenthesin II loses 56% of its activity after 30 d | Nepenthes distillatoria |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2.6 | - |
reaction with acid-denatured haemoglobin, nepenthesin I and II | Nepenthes distillatoria |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Nepenthes distillatoria | nepenthesin II, calculation from nucleotide sequence | - |
3.09 |
Nepenthes distillatoria | nepenthesin Ia and Ib, calculation from nucleotide sequence | - |
3.94 |