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Literature summary for 3.4.23.1 extracted from
- Effect of N-linked glycosylation on the aspartic proteinase porcine pepsin expressed from Pichia pastoris (2004), Glycobiology, 14, 417-429.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E244N/V246T | introduction of N-glycosylation site. Glycosylated mutant shows similar KM-value and substrate specificity as wild-type, but reduced catalytic efficiency | Sus scrofa |
| S110N/L112T | introduction of N-glycosylation site. Glycosylated mutant shows similar KM-value and substrate specificity as wild-type, but reduced catalytic efficiency | Sus scrofa |
| S281N | introduction of N-glycosylation site. Glycosylated mutant shows similar KM-value and substrate specificity as wild-type, but reduced catalytic efficiency | Sus scrofa |
| T77N | introduction of N-glycosylation site. Glycosylated mutant shows similar KM-value and substrate specificity as wild-type, but reduced catalytic efficiency | Sus scrofa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.048 | - |
L-Lys-L-Pro-L-Ala-L-Glu-L-Phe-L-Phe(NO2)-L-Ala-L-Leu | unglycosylated mutant T77N, pH 2.0 | Sus scrofa |  |
| 0.049 | - |
L-Lys-L-Pro-L-Ala-L-Glu-L-Phe-L-Phe(NO2)-L-Ala-L-Leu | unglycosylated mutant S281N, pH 2.0 | Sus scrofa | |
| 0.052 | - |
L-Lys-L-Pro-L-Ala-L-Glu-L-Phe-L-Phe(NO2)-L-Ala-L-Leu | glycosylated mutant T77N, pH 2.0 | Sus scrofa | |
| 0.055 | - |
L-Lys-L-Pro-L-Ala-L-Glu-L-Phe-L-Phe(NO2)-L-Ala-L-Leu | unglycosylated mutant E244N/V246T, pH 2.0 | Sus scrofa | |
| 0.062 | - |
L-Lys-L-Pro-L-Ala-L-Glu-L-Phe-L-Phe(NO2)-L-Ala-L-Leu | wild-type, pH 2.0 | Sus scrofa | |
| 0.064 | - |
L-Lys-L-Pro-L-Ala-L-Glu-L-Phe-L-Phe(NO2)-L-Ala-L-Leu | unglycosylated mutant S110N/L112T, pH 2.0 | Sus scrofa | |
| 0.068 | - |
L-Lys-L-Pro-L-Ala-L-Glu-L-Phe-L-Phe(NO2)-L-Ala-L-Leu | glycosylated mutant E244N/V246T, pH 2.0 | Sus scrofa | |
| 0.069 | - |
L-Lys-L-Pro-L-Ala-L-Glu-L-Phe-L-Phe(NO2)-L-Ala-L-Leu | glycosylated mutant S281N, pH 2.0 | Sus scrofa | |
| 0.075 | - |
L-Lys-L-Pro-L-Ala-L-Glu-L-Phe-L-Phe(NO2)-L-Ala-L-Leu | glycosylated mutant S110N/L112T, pH 2.0 | Sus scrofa | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
expression in Pichia pastoris | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | activation of pepsinogen to pepsin occurs between pH 1.0 and pH 4.0 for wild-type, between pH 1.0 and pH 6.0 for N-glycosylated mutants | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Lys-L-Pro-L-Ala-L-Glu-L-Phe-L-Phe(NO2)-L-Ala-L-Leu + H2O | - |
Sus scrofa | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 38.8 | - |
melting point of wild-type. For glycosylated mutants, melting point increases by 5-17 degrees | Sus scrofa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 16 | - |
L-Lys-L-Pro-L-Ala-L-Glu-L-Phe-L-Phe(NO2)-L-Ala-L-Leu | unglycosylated mutant S110N/L112T, pH 2.0 | Sus scrofa | |
| 53 | - |
L-Lys-L-Pro-L-Ala-L-Glu-L-Phe-L-Phe(NO2)-L-Ala-L-Leu | glycosylated mutant T77N, pH 2.0 | Sus scrofa | |
| 54 | - |
L-Lys-L-Pro-L-Ala-L-Glu-L-Phe-L-Phe(NO2)-L-Ala-L-Leu | glycosylated mutant S110N/L112T, pH 2.0 | Sus scrofa | |
| 68.7 | - |
L-Lys-L-Pro-L-Ala-L-Glu-L-Phe-L-Phe(NO2)-L-Ala-L-Leu | glycosylated mutant S281N, pH 2.0 | Sus scrofa | |
| 70.6 | - |
L-Lys-L-Pro-L-Ala-L-Glu-L-Phe-L-Phe(NO2)-L-Ala-L-Leu | glycosylated mutant E244N/V246T, pH 2.0 | Sus scrofa | |
| 121 | - |
L-Lys-L-Pro-L-Ala-L-Glu-L-Phe-L-Phe(NO2)-L-Ala-L-Leu | unglycosylated mutant E244N/V246T, pH 2.0 | Sus scrofa | |
| 127 | - |
L-Lys-L-Pro-L-Ala-L-Glu-L-Phe-L-Phe(NO2)-L-Ala-L-Leu | unglycosylated mutant S281N, pH 2.0 | Sus scrofa | |
| 150.3 | - |
L-Lys-L-Pro-L-Ala-L-Glu-L-Phe-L-Phe(NO2)-L-Ala-L-Leu | unglycosylated mutant S110N/L112T, pH 2.0 | Sus scrofa | |
| 154 | - |
L-Lys-L-Pro-L-Ala-L-Glu-L-Phe-L-Phe(NO2)-L-Ala-L-Leu | unglycosylated mutant T77N, pH 2.0 | Sus scrofa | |
| 162 | - |
L-Lys-L-Pro-L-Ala-L-Glu-L-Phe-L-Phe(NO2)-L-Ala-L-Leu | wild-type, pH 2.0 | Sus scrofa | |
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