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Literature summary for 3.4.23.1 extracted from

  • Fox, P.F.; Whitaker, J.R.; O'Leary, P.A.
    Isolation and characterization of sheep pepsin (1977), Biochem. J., 161, 389-398.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.23.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.046
-
 N,N-dimethylhemoglobin
-
 Ovis aries
0.05
-
 N,N-dimethylcasein
-
 Ovis aries

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 gel filtration Ovis aries

Organism

Organism UniProt Comment Textmining
Ovis aries
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Ovis aries

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N,N-dimethyl-casein + H2O
-
 Ovis aries ?
-
 ?
N,N-dimethylcasein + H2O
-
 Ovis aries ?
-
 ?
N,N-dimethylhemoglobin + H2O
-
 Ovis aries ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7
-
 N,N-dimethylcasein
-
 Ovis aries
18.3
-
 N,N-dimethylhemoglobin
-
 Ovis aries

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8
-
 N,N-dimethylcasein, N,N-dimethylhemoglobin Ovis aries
1.85
-
 N-acetyl-L-phenylalanyl-L-diiodotyrosine Ovis aries
4.6
-
 benzyloxycarbonyl-L-histidyl-L-phenylalanyl-L-tryptophan ethyl ester Ovis aries

pH Range

pH Minimum pH Maximum Comment Organism
0.5 3.5 N,N-dimethylhemoglobin, pH 0.5: about 30% of maximum activity, pH 3.5: about 15% of maximum activity Ovis aries
0.5 5 N,N-dimethylcasein, pH 0.5: about 35% of maximum activity, pH 5.0: about 20% of maximum activity Ovis aries

pH Stability

pH Stability pH Stability Maximum Comment Organism
1 4.5 24 h, 20°C Ovis aries
7.5
-
 rapid denaturation Ovis aries