Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | stimulates | Porphyromonas gingivalis | |
cysteine | stimulates | Porphyromonas gingivalis | |
dithiothreitol | stimulates | Porphyromonas gingivalis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Porphyromonas gingivalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | inhibitory concentration: 15-20 mM | Porphyromonas gingivalis | |
Co2+ | inhibitory below 5 mM | Porphyromonas gingivalis | |
Cu2+ | inhibitory below 5 mM | Porphyromonas gingivalis | |
EDTA | - |
Porphyromonas gingivalis | |
Fe2+ | inhibitory below 5 mM | Porphyromonas gingivalis | |
Hg2+ | inhibitory below 5 mM | Porphyromonas gingivalis | |
Mg2+ | inhibitory concentration: 15-20 mM | Porphyromonas gingivalis | |
Mn2+ | inhibitory concentration: 5-10 mM | Porphyromonas gingivalis | |
NEM | - |
Porphyromonas gingivalis | |
Ni2+ | inhibitory below 5 mM | Porphyromonas gingivalis | |
PCMB | - |
Porphyromonas gingivalis | |
TLCK | - |
Porphyromonas gingivalis | |
TPCK | - |
Porphyromonas gingivalis | |
Zn2+ | inhibitory below 5 mM | Porphyromonas gingivalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64000 | - |
x * 64000, SDS-PAGE | Porphyromonas gingivalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Porphyromonas gingivalis | the protease constitutes an important virulence factor | ? | - |
? | |
additional information | Porphyromonas gingivalis W83 | the protease constitutes an important virulence factor | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Porphyromonas gingivalis | P25806 | W83 | - |
Porphyromonas gingivalis W83 | P25806 | W83 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Azocoll + H2O | - |
Porphyromonas gingivalis | ? | - |
? | |
Azocoll + H2O | - |
Porphyromonas gingivalis W83 | ? | - |
? | |
Bovine serum albumin + H2O | - |
Porphyromonas gingivalis | ? | - |
? | |
Bovine serum albumin + H2O | - |
Porphyromonas gingivalis W83 | ? | - |
? | |
casein + H2O | - |
Porphyromonas gingivalis | ? | - |
? | |
casein + H2O | - |
Porphyromonas gingivalis W83 | ? | - |
? | |
denatured collagen + H2O | no activity on native collagen | Porphyromonas gingivalis | ? | - |
? | |
additional information | no activity on synthetic peptides such as BAPNA and Gly-Pro-nitroanilide | Porphyromonas gingivalis | ? | - |
? | |
additional information | the protease constitutes an important virulence factor | Porphyromonas gingivalis | ? | - |
? | |
additional information | no activity on synthetic peptides such as BAPNA and Gly-Pro-nitroanilide | Porphyromonas gingivalis W83 | ? | - |
? | |
additional information | the protease constitutes an important virulence factor | Porphyromonas gingivalis W83 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 64000, SDS-PAGE | Porphyromonas gingivalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 70 | - |
Porphyromonas gingivalis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
inactive at or below 20 C when azocoll is the substrate, inactive at 90 C | Porphyromonas gingivalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 12 | - |
Porphyromonas gingivalis |