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Literature summary for 3.4.22.B8 extracted from

  • Bourgeau, G.; Lapointe, H.; Peloquin, P.; Mayrand, D.
    Cloning, expression, and sequencing of a protease gene (tpr) from Porphyromonas gingivalis W83 in Escherichia coli (1992), Infect. Immun., 60, 3186-3192.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.B8

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol stimulates Porphyromonas gingivalis
cysteine stimulates Porphyromonas gingivalis
dithiothreitol stimulates Porphyromonas gingivalis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Porphyromonas gingivalis

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ inhibitory concentration: 15-20 mM Porphyromonas gingivalis
Co2+ inhibitory below 5 mM Porphyromonas gingivalis
Cu2+ inhibitory below 5 mM Porphyromonas gingivalis
EDTA
-
 Porphyromonas gingivalis
Fe2+ inhibitory below 5 mM Porphyromonas gingivalis
Hg2+ inhibitory below 5 mM Porphyromonas gingivalis
Mg2+ inhibitory concentration: 15-20 mM Porphyromonas gingivalis
Mn2+ inhibitory concentration: 5-10 mM Porphyromonas gingivalis
NEM
-
 Porphyromonas gingivalis
Ni2+ inhibitory below 5 mM Porphyromonas gingivalis
PCMB
-
 Porphyromonas gingivalis
TLCK
-
 Porphyromonas gingivalis
TPCK
-
 Porphyromonas gingivalis
Zn2+ inhibitory below 5 mM Porphyromonas gingivalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
 x * 64000, SDS-PAGE Porphyromonas gingivalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Porphyromonas gingivalis the protease constitutes an important virulence factor ?
-
 ?
additional information Porphyromonas gingivalis W83 the protease constitutes an important virulence factor ?
-
 ?

Organism

Organism UniProt Comment Textmining
Porphyromonas gingivalis P25806 W83
-
Porphyromonas gingivalis W83 P25806 W83
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Azocoll + H2O
-
 Porphyromonas gingivalis ?
-
 ?
Azocoll + H2O
-
 Porphyromonas gingivalis W83 ?
-
 ?
Bovine serum albumin + H2O
-
 Porphyromonas gingivalis ?
-
 ?
Bovine serum albumin + H2O
-
 Porphyromonas gingivalis W83 ?
-
 ?
casein + H2O
-
 Porphyromonas gingivalis ?
-
 ?
casein + H2O
-
 Porphyromonas gingivalis W83 ?
-
 ?
denatured collagen + H2O no activity on native collagen Porphyromonas gingivalis ?
-
 ?
additional information no activity on synthetic peptides such as BAPNA and Gly-Pro-nitroanilide Porphyromonas gingivalis ?
-
 ?
additional information the protease constitutes an important virulence factor Porphyromonas gingivalis ?
-
 ?
additional information no activity on synthetic peptides such as BAPNA and Gly-Pro-nitroanilide Porphyromonas gingivalis W83 ?
-
 ?
additional information the protease constitutes an important virulence factor Porphyromonas gingivalis W83 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 64000, SDS-PAGE Porphyromonas gingivalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45 70
-
 Porphyromonas gingivalis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
 inactive at or below 20 C when azocoll is the substrate, inactive at 90 C Porphyromonas gingivalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 12
-
 Porphyromonas gingivalis