Crystallization (Comment) | Organism |
---|---|
structure of nonstructural protein P23 in a precleavage form. The proteins form an extensive interface and nsP3 creates a ring structure that encircles nsP2. The P2/3 cleavage site is located at the base of a narrow cleft and is not readily accessible. The nsP2 protease active site is over 40 A away from the P2/3 cleavage site, supporting a trans cleavage mechanism. nsP3 contains a previously uncharacterized protein fold with a zinc-coordination site. Known mutations in nsP2 that result in formation of noncytopathic viruses or a temperature sensitive phenotype cluster at the nsP2/nsP3 interface | Sindbis virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sindbis virus | P03317 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | nonstructural proteins nsP1-4 are produced as a single polyprotein, and processing of the polyprotein occurs in a highly regulated manner. The P2/3 cleavage site is located at the base of a narrow cleft and is not readily accessible. The nsP2 protease active site is over 40 A away from the P2/3 cleavage site, supporting a trans cleavage mechanism | Sindbis virus |