Cloned (Comment) | Organism |
---|---|
the gene that encodes a double-mutant IBV nsp2 N-terminal domain (residues 9-393 of the polyprotein, with mutations Q132L and L270F) is expressed in Escherichia coli BL21(DE3) cells | infectious bronchitis virus |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 12%(w/v) PEG 8000, 10%(v/v) ethylene glycol, 100 mM HEPES pH 7.5 | infectious bronchitis virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
infectious bronchitis virus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
GST-glutathione affinity column chromatography | infectious bronchitis virus |
Synonyms | Comment | Organism |
---|---|---|
nonstructural protein 2 | - |
infectious bronchitis virus |
nsp2 protein | - |
infectious bronchitis virus |