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Literature summary for 3.4.22.B71 extracted from

  • Alegre, K.O.; Reverter, D.
    Structural insights into the SENP6 loop1 structure in complex with SUMO2 (2014), Protein Sci., 23, 433-441.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.B71

Crystallization (Commentary)

Crystallization (Comment) Organism
recombinant chimeric mutant SENP2 C548S-loop1 in complex with SUMO2, X-ray diffraction structure determination and analysis at 2.15 A resolution Homo sapiens

Protein Variants

Protein Variants Comment Organism
C548S site-directed mutagenesis, active site mutant Homo sapiens
F393G/K394G construction of a chimeric enzyme SENP2 mutant with the insertion of loop1 from SENP6 into its sequence causing mutation of F393G/K394G, loop1 insertion is a determinant for SUMO2/3 activity and specificity, structure of SENP2-loop1 in complex with SUMO2, overview. The mutant reveals the details of an interface exclusive to SENP6/7 and the formation of unique contacts between both proteins, and the mutant shows an increase of the proteolytic activity for diSUMO2 and polySUMO2 substrates. The chimeric insertion mutant used for crystallization studies is also mutated at active site residue, C548S, for a stable complex formation Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9HC62
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant chimeric mutant SENP2 C548S-loop1 in complex with SUMO2 by gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diSUMO2 + H2O
-
 Homo sapiens 2 SUMO2
-
 ?
polySUMO2 + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
SENP2
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Homo sapiens

General Information

General Information Comment Organism
evolution the enzyme belongs to the SENP/ULP protease family Homo sapiens