Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
Senp2 crystallization is performed at 4°C using sitting and hanging drop vapor diffusion methods. X-ray structures of Senp2 catalytic protease domain and of a covalent thiohemiacetal transition-state complex obtained between the Senp2 catalytic domain and SUMO-1 reveales details of the respective protease and substrate surfaces utilized in interactions between these two proteins. Comparative biochemical and structural analysis between Senp2 and the yeast SUMO protease Ulp1 reveales differential abilities to process SUMO-1, SUMO-2, and SUMO-3 in maturation and deconjugation reactions | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9HC62 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
testis | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(SUMO-1)-Ran GTPase-activating protein 1 conjugate + H2O | - |
Homo sapiens | SUMO-1 + Ran GTPase-activating protein 1 | - |
? | |
(SUMO-2)-Ran GTPase-activating protein 1 conjugate + H2O | - |
Homo sapiens | SUMO-2 + Ran GTPase-activating protein 1 | - |
? | |
(SUMO-3)-Ran GTPase-activating protein 1 conjugate + H2O | - |
Homo sapiens | SUMO-3 + Ran GTPase-activating protein 1 | - |
? | |
SUMO-1 precursor + H2O | 50% of SUMO-1 precursor is hydrolyzed after 70 min | Homo sapiens | SUMO-1 + His-Ser-Thr-Val | - |
? | |
SUMO-2 precursor + H2O | 50% of SUMO-2 precursor is hydrolyzed after 15 min | Homo sapiens | SUMO-2 + Val-Tyr | - |
? | |
SUMO-3 precursor + H2O | 50% of SUMO-1 precursor is hydrolyzed after 160 min | Homo sapiens | SUMO-3 + Val-Pro-Glu-Ser-Ser-Leu-Ala-Gly-His-Ser-Phe | - |
? |