Cloned (Comment) | Organism |
---|---|
crystallization of a complex of SENP1 C603A bound to full-length SUMO-1 and determination of the structure by X-ray crystallography. Two structures of SENP1 C603A in complex with the SUMO-1 precursor and RanGAP1-SUMO-1 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
SUMO-1-GG | product inhibition, competitive, inhibits cleavage of the SUMO-1 precursor, SUMO-2 precursor, (SUMO-1)-RanGAP1 conjugate and (SUMO-2)-RanGAP1 conjugate | Homo sapiens | |
SUMO-2-GG | product inhibition, competitive, inhibits cleavage of the SUMO-1 precursor, SUMO-2 precursor, (SUMO-1)-RanGAP1 conjugate and (SUMO-2)-RanGAP1 conjugate | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000018 | - |
SUMO-1 precursor | pH 7.5, 30°C | Homo sapiens | |
0.00000198 | - |
SUMO-2 precursor | pH 7.5, 30°C | Homo sapiens | |
0.00000224 | - |
(SUMO-1)-RanGAP1 conjugate | pH 7.5, 30°C | Homo sapiens | |
0.00000275 | - |
(SUMO-2)-RanGAP1 conjugate | pH 7.5, 30°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9P0U3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(SUMO-1)-RanGAP1 conjugate + H2O | SENP1 binding is accompanied by a conformational change in the substrate | Homo sapiens | SUMO-1 + RanGAP1 | - |
? | |
(SUMO-2)-RanGAP1 conjugate + H2O | SENP1 binding is accompanied by a conformational change in the substrate | Homo sapiens | SUMO-2 + RanGAP1 | - |
? | |
SUMO-1 precursor + H2O | SENP1 preferentially processes SUMO-1 over SUMO-2. Discrimination between unprocessed SUMO-1 and SUMO-2 by SENP1 is based on a catalytic step rather than substrate binding. SENP1 binding is accompanied by a conformational change in the substrate | Homo sapiens | SUMO-1 + His-Ser-Thr-Val | - |
? | |
SUMO-2 precursor + H2O | SENP1 preferentially processes SUMO-1 over SUMO-2. Discrimination between unprocessed SUMO-1 and SUMO-2 by SENP1 is based on a catalytic step rather than substrate binding. SENP1 binding is accompanied by a conformational change in the substrate | Homo sapiens | SUMO-2 + Val-Tyr | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SENP1 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.075 | - |
SUMO-2 precursor | pH 7.5, 30°C | Homo sapiens | |
3.7 | - |
SUMO-1 precursor | pH 7.5, 30°C | Homo sapiens | |
8.3 | - |
(SUMO-1)-RanGAP1 conjugate | pH 7.5, 30°C | Homo sapiens | |
18.2 | - |
(SUMO-2)-RanGAP1 conjugate | pH 7.5, 30°C | Homo sapiens |