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Literature summary for 3.4.22.B70 extracted from

  • Shen, L.; Tatham, M.H.; Dong, C.; Zagorska, A.; Naismith, J.H.; Hay, R.T.
    SUMO protease SENP1 induces isomerization of the scissile peptide bond (2006), Nat. Struct. Mol. Biol., 13, 1069-1077.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
crystallization of a complex of SENP1 C603A bound to full-length SUMO-1 and determination of the structure by X-ray crystallography. Two structures of SENP1 C603A in complex with the SUMO-1 precursor and RanGAP1-SUMO-1 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
SUMO-1-GG product inhibition, competitive, inhibits cleavage of the SUMO-1 precursor, SUMO-2 precursor, (SUMO-1)-RanGAP1 conjugate and (SUMO-2)-RanGAP1 conjugate Homo sapiens
SUMO-2-GG product inhibition, competitive, inhibits cleavage of the SUMO-1 precursor, SUMO-2 precursor, (SUMO-1)-RanGAP1 conjugate and (SUMO-2)-RanGAP1 conjugate Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0000018
-
SUMO-1 precursor pH 7.5, 30°C Homo sapiens
0.00000198
-
SUMO-2 precursor pH 7.5, 30°C Homo sapiens
0.00000224
-
(SUMO-1)-RanGAP1 conjugate pH 7.5, 30°C Homo sapiens
0.00000275
-
(SUMO-2)-RanGAP1 conjugate pH 7.5, 30°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9P0U3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(SUMO-1)-RanGAP1 conjugate + H2O SENP1 binding is accompanied by a conformational change in the substrate Homo sapiens SUMO-1 + RanGAP1
-
?
(SUMO-2)-RanGAP1 conjugate + H2O SENP1 binding is accompanied by a conformational change in the substrate Homo sapiens SUMO-2 + RanGAP1
-
?
SUMO-1 precursor + H2O SENP1 preferentially processes SUMO-1 over SUMO-2. Discrimination between unprocessed SUMO-1 and SUMO-2 by SENP1 is based on a catalytic step rather than substrate binding. SENP1 binding is accompanied by a conformational change in the substrate Homo sapiens SUMO-1 + His-Ser-Thr-Val
-
?
SUMO-2 precursor + H2O SENP1 preferentially processes SUMO-1 over SUMO-2. Discrimination between unprocessed SUMO-1 and SUMO-2 by SENP1 is based on a catalytic step rather than substrate binding. SENP1 binding is accompanied by a conformational change in the substrate Homo sapiens SUMO-2 + Val-Tyr
-
?

Synonyms

Synonyms Comment Organism
SENP1
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.075
-
SUMO-2 precursor pH 7.5, 30°C Homo sapiens
3.7
-
SUMO-1 precursor pH 7.5, 30°C Homo sapiens
8.3
-
(SUMO-1)-RanGAP1 conjugate pH 7.5, 30°C Homo sapiens
18.2
-
(SUMO-2)-RanGAP1 conjugate pH 7.5, 30°C Homo sapiens