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Literature summary for 3.4.22.B67 extracted from

  • Li, S.J.; Hochstrasser, M.
    The yeast ULP2 (SMT4) gene encodes a novel protease specific for the ubiquitin-like Smt3 protein (2000), Mol. Cell. Biol., 20, 2367-2377.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.B67

Cloned(Commentary)

Cloned (Comment) Organism
expression as a GST-Ulp2 fusion protein by Escherichia coli Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by phenylmethylsulfonyl fluoride or the Dub-specific inhibitor ubiquitin aldehyde Saccharomyces cerevisiae
N-ethylmaleimide
-
 Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Saccharomyces cerevisiae 5634
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Smt3-modified protein conjugate + H2O Saccharomyces cerevisiae Ulp2 is able to cleave isopeptide-linked Smt3 molecules from natural substrates. Smt3-protein deconjugation by Ulp2 is important for normal meiotic development Smt3 + protein
-
 ?
Smt3-precursor + H2O Saccharomyces cerevisiae Ulp2 is able to process the Smt3 precursor in vivo, albeit inefficiently relative to Ulp1 SMT3 + peptide
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P40537
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Smt3-modified protein conjugate + H2O Ulp2 is able to cleave isopeptide-linked Smt3 molecules from natural substrates. Smt3-protein deconjugation by Ulp2 is important for normal meiotic development Saccharomyces cerevisiae Smt3 + protein
-
 ?
Smt3-modified protein conjugate + H2O Ulp2 is able to cleave isopeptide-linked Smt3 molecules from natural substrates Saccharomyces cerevisiae Smt3 + protein
-
 ?
Smt3-precursor + H2O Ulp2 is able to process the Smt3 precursor in vivo, albeit inefficiently relative to Ulp1 Saccharomyces cerevisiae SMT3 + peptide
-
 ?

Synonyms

Synonyms Comment Organism
SMT4
-
 Saccharomyces cerevisiae
Ulp2
-
 Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction deletion of ULP2 results in a diverse set of aberrations, but the gene is not essential. In cells lacking Ulp2, specific Smt3-protein conjugates accumulate, and the conjugate pattern is distinct from that observed in a ulp1ts strain, which is defective for a distantly related Smt3-specific protease, Ulp1. The ulp2D mutant exhibits a pleiotropic phenotype that includes temperature-sensitive growth, abnormal cell morphology, decreased plasmid and chromosome stability, and a severe sporulation defect. The mutant is also hypersensitive to DNA damaging agents, hydroxyurea, and benomyl Saccharomyces cerevisiae