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Literature summary for 3.4.22.B63 extracted from

  • Cozzi, R.; Prigozhin, D.; Rosini, R.; Abate, F.; Bottomley, M.J.; Grandi, G.; Telford, J.L.; Rinaudo, C.D.; Maione, D.; Alber, T.
    Structural basis for group B Streptococcus pilus 1 sortases C regulation and specificity (2012), PLoS ONE, 7, e49048.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.B63

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3) pLysS cells Streptococcus agalactiae serogroup V

Crystallization (Commentary)

Crystallization (Comment) Organism
isofofm SrtC1, hanging drop vapor diffusion method, using 0.4 M sodium formate, 0.1 M Bis-Tris propane pH 6.5, and 22% (w/v) PEG 3350. Isoform SrtC1, hanging drop vapor diffusion method, using 0.26 M CaCl2, 19% (w/v) PEG 6000, 0.1 M HEPES pH 7.0 Streptococcus agalactiae serogroup V

Protein Variants

Protein Variants Comment Organism
F86A the mutant shows decreased catalytic efficiency compared to the wild type Streptococcus agalactiae serogroup V
Y92A the mutant shows increased catalytic efficiency compared to the wild type Streptococcus agalactiae serogroup V

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00358
-
 Dabcyl-ancillary protein 1-Edans wild type isoform SrtC1, at pH 7.5 and 37°C Streptococcus agalactiae serogroup V
0.00569
-
 Dabcyl-ancillary protein 1-Edans Y92A mutant isoform SrtC1, at pH 7.5 and 37°C Streptococcus agalactiae serogroup V
0.006385
-
 Dabcyl-ancillary protein 1-Edans wild type isoform SrtC2, at pH 7.5 and 37°C Streptococcus agalactiae serogroup V
0.01421
-
 Dabcyl-ancillary protein 2-Edans Y92A mutant isoform SrtC1, at pH 7.5 and 37°C Streptococcus agalactiae serogroup V
0.01639
-
 Dabcyl-ancillary protein 2-Edans wild type isoform SrtC1, at pH 7.5 and 37°C Streptococcus agalactiae serogroup V
0.01814
-
 Dabcyl-ancillary protein 2-Edans F86A mutant isoform SrtC2, at pH 7.5 and 37°C Streptococcus agalactiae serogroup V
0.02156
-
 Dabcyl-BP-1 peptide-Edans wild type isoform SrtC2, at pH 7.5 and 37°C Streptococcus agalactiae serogroup V
0.02733
-
 Dabcyl-ancillary protein 2-Edans wild type isoform SrtC2, at pH 7.5 and 37°C Streptococcus agalactiae serogroup V
0.031
-
 Dabcyl-BP-1 peptide-Edans wild type isoform SrtC1, at pH 7.5 and 37°C Streptococcus agalactiae serogroup V
0.03235
-
 Dabcyl-BP-1 peptide-Edans Y92A mutant isoform SrtC1, at pH 7.5 and 37°C Streptococcus agalactiae serogroup V
0.04385
-
 Dabcyl-BP-1 peptide-Edans F86A mutant isoform SrtC2, at pH 7.5 and 37°C Streptococcus agalactiae serogroup V
0.06036
-
 Dabcyl-ancillary protein 1-Edans F86A mutant isoform SrtC2, at pH 7.5 and 37°C Streptococcus agalactiae serogroup V

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Dabcyl-ancillary protein 1-Edans + H2O Streptococcus agalactiae serogroup V preferred substrate of SrtC2 ?
-
 ?
Dabcyl-ancillary protein 1-Edans + H2O Streptococcus agalactiae serogroup V ATCC BAA-611 preferred substrate of SrtC2 ?
-
 ?
Dabcyl-ancillary protein 2-Edans + H2O Streptococcus agalactiae serogroup V preferred substrate of SrtC1 ?
-
 ?
Dabcyl-ancillary protein 2-Edans + H2O Streptococcus agalactiae serogroup V ATCC BAA-611 preferred substrate of SrtC1 ?
-
 ?
Dabcyl-BP-1 peptide-Edans + H2O Streptococcus agalactiae serogroup V
-
 ?
-
 ?
Dabcyl-BP-1 peptide-Edans + H2O Streptococcus agalactiae serogroup V ATCC BAA-611
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Streptococcus agalactiae serogroup V Q8E0S7 group B
-
Streptococcus agalactiae serogroup V ATCC BAA-611 Q8E0S7 group B
-

Purification (Commentary)

Purification (Comment) Organism
His-Trap column chromatography, and Superdex 75 gel filtration Streptococcus agalactiae serogroup V

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Dabcyl-ancillary protein 1-Edans + H2O preferred substrate of SrtC2 Streptococcus agalactiae serogroup V ?
-
 ?
Dabcyl-ancillary protein 1-Edans + H2O preferred substrate of SrtC2 Streptococcus agalactiae serogroup V ATCC BAA-611 ?
-
 ?
Dabcyl-ancillary protein 2-Edans + H2O preferred substrate of SrtC1 Streptococcus agalactiae serogroup V ?
-
 ?
Dabcyl-ancillary protein 2-Edans + H2O preferred substrate of SrtC1 Streptococcus agalactiae serogroup V ATCC BAA-611 ?
-
 ?
Dabcyl-BP-1 peptide-Edans + H2O
-
 Streptococcus agalactiae serogroup V ?
-
 ?
Dabcyl-BP-1 peptide-Edans + H2O
-
 Streptococcus agalactiae serogroup V ATCC BAA-611 ?
-
 ?

Subunits

Subunits Comment Organism
monomer gel filtration Streptococcus agalactiae serogroup V

Synonyms

Synonyms Comment Organism
sortase C1
-
 Streptococcus agalactiae serogroup V
sortase C2
-
 Streptococcus agalactiae serogroup V
SrtC1 isoform Streptococcus agalactiae serogroup V
SrtC2 isoform Streptococcus agalactiae serogroup V

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00041
-
 Dabcyl-ancillary protein 2-Edans F86A mutant isoform SrtC2, at pH 7.5 and 37°C Streptococcus agalactiae serogroup V
0.000436
-
 Dabcyl-ancillary protein 2-Edans wild type isoform SrtC2, at pH 7.5 and 37°C Streptococcus agalactiae serogroup V
0.000731
-
 Dabcyl-BP-1 peptide-Edans wild type isoform SrtC2, at pH 7.5 and 37°C Streptococcus agalactiae serogroup V
0.00077
-
 Dabcyl-ancillary protein 2-Edans wild type isoform SrtC1, at pH 7.5 and 37°C Streptococcus agalactiae serogroup V
0.00104
-
 Dabcyl-ancillary protein 1-Edans wild type isoform SrtC2, at pH 7.5 and 37°C Streptococcus agalactiae serogroup V
0.00106
-
 Dabcyl-BP-1 peptide-Edans F86A mutant isoform SrtC2, at pH 7.5 and 37°C Streptococcus agalactiae serogroup V
0.00113
-
 Dabcyl-ancillary protein 2-Edans Y92A mutant isoform SrtC1, at pH 7.5 and 37°C Streptococcus agalactiae serogroup V
0.00116
-
 Dabcyl-ancillary protein 1-Edans wild type isoform SrtC1, at pH 7.5 and 37°C Streptococcus agalactiae serogroup V
0.00177
-
 Dabcyl-BP-1 peptide-Edans wild type isoform SrtC1, at pH 7.5 and 37°C Streptococcus agalactiae serogroup V
0.00216
-
 Dabcyl-ancillary protein 1-Edans Y92A mutant isoform SrtC1, at pH 7.5 and 37°C Streptococcus agalactiae serogroup V
0.00298
-
 Dabcyl-BP-1 peptide-Edans Y92A mutant isoform SrtC1, at pH 7.5 and 37°C Streptococcus agalactiae serogroup V
0.0049
-
 Dabcyl-ancillary protein 1-Edans F86A mutant isoform SrtC2, at pH 7.5 and 37°C Streptococcus agalactiae serogroup V

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
16.2
-
 Dabcyl-ancillary protein 1-Edans wild type isoform SrtC2, at pH 7.5 and 37°C Streptococcus agalactiae serogroup V
32.5
-
 Dabcyl-ancillary protein 1-Edans wild type isoform SrtC1, at pH 7.5 and 37°C Streptococcus agalactiae serogroup V
38.1
-
 Dabcyl-ancillary protein 1-Edans Y92A mutant isoform SrtC1, at pH 7.5 and 37°C Streptococcus agalactiae serogroup V
159
-
 Dabcyl-ancillary protein 2-Edans wild type isoform SrtC2, at pH 7.5 and 37°C Streptococcus agalactiae serogroup V
226
-
 Dabcyl-ancillary protein 2-Edans F86A mutant isoform SrtC2, at pH 7.5 and 37°C Streptococcus agalactiae serogroup V
243
-
 Dabcyl-BP-1 peptide-Edans F86A mutant isoform SrtC2, at pH 7.5 and 37°C Streptococcus agalactiae serogroup V
339
-
 Dabcyl-BP-1 peptide-Edans wild type isoform SrtC2, at pH 7.5 and 37°C Streptococcus agalactiae serogroup V
470
-
 Dabcyl-ancillary protein 2-Edans wild type isoform SrtC1, at pH 7.5 and 37°C Streptococcus agalactiae serogroup V
570
-
 Dabcyl-BP-1 peptide-Edans wild type isoform SrtC1, at pH 7.5 and 37°C Streptococcus agalactiae serogroup V
797
-
 Dabcyl-ancillary protein 2-Edans Y92A mutant isoform SrtC1, at pH 7.5 and 37°C Streptococcus agalactiae serogroup V
810
-
 Dabcyl-ancillary protein 1-Edans F86A mutant isoform SrtC2, at pH 7.5 and 37°C Streptococcus agalactiae serogroup V
922
-
 Dabcyl-BP-1 peptide-Edans Y92A mutant isoform SrtC1, at pH 7.5 and 37°C Streptococcus agalactiae serogroup V