Literature summary for 3.4.22.B63 extracted from

Cozzi, R.; Malito, E.; Nuccitelli, A.; DOnofrio, M.; Martinelli, M.; Ferlenghi, I.; Grandi, G.; Telford, J.L.; Maione, D.; Rinaudo, C.D.
Structure analysis and site-directed mutagenesis of defined key residues and motives for pilus-related sortase C1 in group B Streptococcus (2011), FASEB J., 25, 1874-1886.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 100 mM Tris (pH 8.5), 200 mM NaOAc, and 30% (w/v) PEG-4000	Streptococcus agalactiae

Protein Variants

Protein Variants	Comment	Organism
C219A/H157A/R228A	inactive	Streptococcus agalactiae
D84A	the mutation in the lid does not abrogate pilus protein polymerization	Streptococcus agalactiae
Y86A	the mutant show an increased Km value compared to the wild type enzyme, the mutation in the lid does not abrogate pilus protein polymerization	Streptococcus agalactiae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0138	-	Dabcyl-KKVTIPQTGGIGT-EDANS	wild type enzyme, at 37°C in 20 mM HEPES (pH 7.5), 75 mM NaCl, and 1 mM dithiothreitol	Streptococcus agalactiae
0.0294	-	Dabcyl-KKVTIPQTGGIGT-EDANS	mutant enzyme Y86A, at 37°C in 20 mM HEPES (pH 7.5), 75 mM NaCl, and 1 mM dithiothreitol	Streptococcus agalactiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Streptococcus agalactiae	16020	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	SrtC1 is not involved in calcium binding	Streptococcus agalactiae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
28000	-	x * 28000, SDS-PAGE	Streptococcus agalactiae

Organism

Organism	UniProt	Comment	Textmining
Streptococcus agalactiae	Q69EY7	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Dabcyl-KKVTIPQTGGIGT-EDANS + H2O	-	Streptococcus agalactiae	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 28000, SDS-PAGE	Streptococcus agalactiae

Synonyms

Synonyms	Comment	Organism
sortase C1	-	Streptococcus agalactiae
SrtC1	-	Streptococcus agalactiae

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Release 2023.1 (January 2023)