Literature summary for 3.4.22.B60 extracted from

Berasain, P.; Carmona, C.; Frangione, B.; Dalton, J.P.; Goni, F.
Fasciola hepatica: parasite-secreted proteinases degrade all human IgG subclasses: determination of the specific cleavage sites and identification of the immunoglobulin fragments produced (2000), Exp. Parasitol., 94, 99-110.

Search for more literature for 3.4.22.B60

Activating Compound

Activating Compound	Comment	Organism	Structure
dithiothreitol	the cathepsins L needed the presence of dithiothreitol to digest IgG1, IgG2, and IgG4 whereas IgG3 was identically cleaved under both reducing and nonreducing conditions	Fasciola hepatica

Organism

Organism	UniProt	Comment	Textmining
Fasciola hepatica	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Fasciola hepatica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
16.2	-	substrate: tosyl-Gly-Pro-Arg-4-methylcoumarinyl-7-amide	Fasciola hepatica

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
human IgG + H2O	both cathepsins L produce similar degradation patterns and cleave all human IgG subclasses at the hinge region, yielding at pH 7.3 and 37°C Fab and Fc fragments in the case of IgG1 and IgG3 or Fab(2) and Fc in IgG2 and IgG4. Both liver fluke cathepsins L cleave the peptide bonds 237His-Thr, 237Glu-Cys, 233Gly-Asp, and 241Ser-Cys of the gamma1, gamma2, gamma3, and gamma4 H chains, respectively. Therefore, the enzymes are interacting with the following P3-P'3 sequences, Lys-Thr-His-Thr-Cys-Pro, Cys-Val-Glu-Asp-Pro-Pro, Pro-Leu-Gly-Asp-Thr-Thr, and Cys-Pro-Ser-Cys-Pro-Ala. The specificity of the liver fluke cathepsins L for peptide bonds in proteins is less defined. The P1 position, for instance, can be occupied by hydrophobic, hydrophilic, acidic, or basic residues. The P3 and P2 positions are occupied by hydrophobic amino acids with the exception of the gamma1 sequence which contains a basic lysine and a hydrophilic threonine, respectively. In addition the specificity between the enzyme and its substrate would depend on which of the amino acids of the substrate can be really exposed to the active site	Fasciola hepatica	?	-	?
tosyl-Gly-Pro-Arg-4-methylcoumarinyl-7-amide + H2O	-	Fasciola hepatica	tosyl-Gly-Pro-Arg + 7-amino-4-methylcoumarin	-	?

Synonyms

Synonyms	Comment	Organism
cathepsin L2	-	Fasciola hepatica
CL2	-	Fasciola hepatica

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)