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Literature summary for 3.4.22.B60 extracted from

  • Dowd, A.J.; Smith, A.M.; McGonigle, S.; Dalton, J.P.
    Purification and characterization of a second cathepsin L proteinase secreted by parasitic trematode Fasciola hepatica (1994), Eur. J. Biochem., 223, 91-98.
    View publication on PubMed
Search for more literature for 3.4.22.B60

Inhibitors

Inhibitors Comment Organism Structure
Tetranitromethane cathepsin L2 is completely inactivated by 4 mM tetranitromethane, cathepsin L1 is not inactivated Fasciola hepatica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
 benzoyl-Phe-Val-Arg-4-methylcoumarinyl-7-amide pH 7.0, 37°C, cathepsin L2 Fasciola hepatica
0.0038
-
 Leu-Val-Tyr-4-methylcoumarinyl-7-amide pH 7.0, 37°C, cathepsin L2 Fasciola hepatica
0.004
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarinyl-7-amide pH 7.0, 37°C, cathepsin L2 Fasciola hepatica
0.0073
-
 tert-butyloxycarbonyl-Val-Leu-Lys-4-methylcoumarinyl-7-amide pH 7.0, 37°C, cathepsin L2 Fasciola hepatica
0.0091
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumarinyl-7-amide pH 7.0, 37°C, cathepsin L2 Fasciola hepatica
0.0141
-
 benzyloxycarbonyl-Arg-4-methylcoumarinyl-7-amide pH 7.0, 37°C, cathepsin L2 Fasciola hepatica
0.0171
-
 tosyl-Gly-Pro-Arg-4-methylcoumarinyl-7-amide pH 7.0, 37°C, cathepsin L2 Fasciola hepatica
0.0232
-
 succinyl-Leu-Leu-Val-Tyr-4-methylcoumarinyl-7-amide pH 7.0, 37°C, cathepsin L2 Fasciola hepatica
0.0248
-
 tert-butyloxycarbonyl-Val-Pro-Arg-4-methylcoumarinyl-7-amide pH 7.0, 37°C, cathepsin L2 Fasciola hepatica
0.0355
-
 tosyl-Gly-Pro-Lys-4-methylcoumarinyl-7-amide pH 7.0, 37°C, cathepsin L2 Fasciola hepatica
0.0412
-
 succinyl-Ala-Phe-Lys-4-methylcoumarinyl-7-amide pH 7.0, 37°C, cathepsin L2 Fasciola hepatica

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular secreted by adult flukes Fasciola hepatica
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29500
-
 x * 29500, SDS-Page Fasciola hepatica

Organic Solvent Stability

Organic Solvent Comment Organism
Ethanol 10% v/v, no inactivation of cathepsin L1 or cathepsin L2 Fasciola hepatica

Organism

Organism UniProt Comment Textmining
Fasciola hepatica P80342 fragment
-

Purification (Commentary)

Purification (Comment) Organism
-
 Fasciola hepatica

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Fasciola hepatica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.94
-
-
 Fasciola hepatica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoyl-Phe-Val-Arg-4-methylcoumarinyl-7-amide + H2O cathepsin L2 Fasciola hepatica benzoyl-Phe-Val-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Arg-4-methylcoumarinyl-7-amide + H2O cathepsin L2 Fasciola hepatica benzyloxycarbonyl-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Arg-Arg-4-methylcoumarinyl-7-amide + H2O cathepsin L2 Fasciola hepatica benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Phe-Arg-4-methylcoumarinyl-7-amide + H2O cleaved by cathepsin L2 with much greater affinity than by cathepsin L1 Fasciola hepatica benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
Leu-Val-Tyr-4-methylcoumarinyl-7-amide + H2O cleaved by cathepsin L2 with much greater affinity than by cathepsin L1 Fasciola hepatica Leu-Val-Tyr + 7-amino-4-methylcoumarin
-
 ?
additional information cathepsin L1 and cathepsin L2 proteinases may be the prime mechanism by which the parasite penetrates tissue Fasciola hepatica ?
-
 ?
succinyl-Ala-Phe-Lys-4-methylcoumarinyl-7-amide + H2O cathepsin L2 Fasciola hepatica succinyl-Ala-Phe-Lys + 7-amino-4-methylcoumarin
-
 ?
succinyl-Leu-Leu-Val-Tyr-4-methylcoumarinyl-7-amide + H2O cathepsin L2 Fasciola hepatica succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
-
 ?
tert-butyloxycarbonyl-Val-Leu-Lys-4-methylcoumarinyl-7-amide + H2O cathepsin L2 Fasciola hepatica tert-butyloxycarbonyl-Val-Leu-Lys + 7-amino-4-methylcoumarin
-
 ?
tert-butyloxycarbonyl-Val-Pro-Arg-4-methylcoumarinyl-7-amide + H2O cleaved by cathepsin L2 with much greater affinity than by cathepsin L1 Fasciola hepatica tert-butyloxycarbonyl-Val-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?
tosyl-Ala-Phe-Lys-4-methylcoumarinyl-7-amide + H2O cathepsin L2 Fasciola hepatica tosyl-Ala-Phe-Lys + 7-amino-4-methylcoumarin
-
 ?
tosyl-Gly-Pro-Arg-4-methylcoumarinyl-7-amide + H2O cleaved by cathepsin L2 with much greater affinity than by cathepsin L1 Fasciola hepatica tosyl-Gly-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?
tosyl-Gly-Pro-Lys-4-methylcoumarinyl-7-amide + H2O cleaved by cathepsin L2 with much greater affinity than by cathepsin L1 Fasciola hepatica tosyl-Gly-Pro-Lys + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
? x * 29500, SDS-Page Fasciola hepatica

Synonyms

Synonyms Comment Organism
cathepsin L2
-
 Fasciola hepatica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumarinyl-7-amide pH 7.0, 37°C, cathepsin L2 Fasciola hepatica
0.06
-
 benzoyl-Phe-Val-Arg-4-methylcoumarinyl-7-amide pH 7.0, 37°C, cathepsin L2 Fasciola hepatica
0.08
-
 succinyl-Leu-Leu-Val-Tyr-4-methylcoumarinyl-7-amide pH 7.0, 37°C, cathepsin L2 Fasciola hepatica
0.09
-
 benzyloxycarbonyl-Arg-4-methylcoumarinyl-7-amide pH 7.0, 37°C, cathepsin L2 Fasciola hepatica
0.14
-
 succinyl-Ala-Phe-Lys-4-methylcoumarinyl-7-amide pH 7.0, 37°C, cathepsin L2 Fasciola hepatica
0.21
-
 Leu-Val-Tyr-4-methylcoumarinyl-7-amide pH 7.0, 37°C, cathepsin L2 Fasciola hepatica
0.56
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarinyl-7-amide pH 7.0, 37°C, cathepsin L2 Fasciola hepatica
1.19
-
 tert-butyloxycarbonyl-Val-Pro-Arg-4-methylcoumarinyl-7-amide pH 7.0, 37°C, cathepsin L2 Fasciola hepatica
1.37
-
 tosyl-Gly-Pro-Lys-4-methylcoumarinyl-7-amide pH 7.0, 37°C, cathepsin L2 Fasciola hepatica
1.93
-
 tosyl-Gly-Pro-Arg-4-methylcoumarinyl-7-amide pH 7.0, 37°C, cathepsin L2 Fasciola hepatica
3.75
-
 tert-butyloxycarbonyl-Val-Leu-Lys-4-methylcoumarinyl-7-amide pH 7.0, 37°C, cathepsin L2 Fasciola hepatica