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Literature summary for 3.4.22.B6 extracted from
- Cloning and characterization of a novel cathepsin B-like cysteine proteinase from Angiostrongylus cantonensis (2012), Parasitol. Res., 110, 2413-2422.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cDNA library screening, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Angiostrongylus cantonensis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (1S,2S)-2-(((S)-1-((4-guanidinobutyl)amino)-4-methyl-1-oxopentan-2-yl)carbamoyl)cyclopropanecarboxylic acid | i.e. E-64 | Angiostrongylus cantonensis |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | the excretory/secretory enzyme is synthesized as preproprotein composed of a signal sequence, a proenzyme region , and a mature proteinase sequence | Angiostrongylus cantonensis | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27400 | - |
x * 37800, about, preproprotein, sequence calculation, x * 27400, about, mature protein, sequence calculation | Angiostrongylus cantonensis |
| 37800 | - |
x * 37800, about, preproprotein, sequence calculation, x * 27400, about, mature protein, sequence calculation | Angiostrongylus cantonensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Angiostrongylus cantonensis | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | the enzyme is synthesized as preproprotein composed of a signal sequence, a proenzyme region , and a mature proteinase sequence | Angiostrongylus cantonensis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis | Angiostrongylus cantonensis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| intestine | mainly | Angiostrongylus cantonensis | - |
| larva | fourth-stage larvae, the mature enzyme is an excretory/secretory product of of L4 larval stage | Angiostrongylus cantonensis | - |
| additional information | enzyme expression throughout various developmental stages, L3, L4, adult male and female worms, immunohistochemic analysis | Angiostrongylus cantonensis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hemoglobinase + H2O | degradation | Angiostrongylus cantonensis | ? | - |
? | |
| human IgG + H2O | degradation | Angiostrongylus cantonensis | ? | - |
? | |
| Z-Arg-Arg-7-amido-4-methylcoumarin + H2O | - |
Angiostrongylus cantonensis | Z-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 37800, about, preproprotein, sequence calculation, x * 27400, about, mature protein, sequence calculation | Angiostrongylus cantonensis |
| More | the preproprotein is composed of a signal sequence of 17 residues, a proenzyme region of 76 amino acids, and a mature proteinase sequence of 251 amino acids in length | Angiostrongylus cantonensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cathepsin B-like cysteine proteinase | - |
Angiostrongylus cantonensis |
| CBL1 | - |
Angiostrongylus cantonensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Angiostrongylus cantonensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Angiostrongylus cantonensis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Angiostrongylus cantonensis | sequence calculation, mature protein | - |
4.89 |
| Angiostrongylus cantonensis | sequence calculation, preproprotein | - |
5.42 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme is related to other cathepsin B family members with the conserved catalytic triad (Cys, His, Asn) and diagnostic occluding loop | Angiostrongylus cantonensis |
| additional information | the conserved residues Cys122, His292, and Asn312 form the typical cysteine protease catalytic triad. The enzyme sequence contains a specific hemoglobinase motif and might have a hemoglobinase -degrading function | Angiostrongylus cantonensis |
| physiological function | the mature enzyme is an excretory/secretory product of of L4 larval stage of Angiostrongylus cantonensis, excretory-secretory antigens are involved in parasite-host interactions | Angiostrongylus cantonensis |
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Release 2023.1 (January 2023)
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