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Literature summary for 3.4.22.B6 extracted from

  • Kerr, I.D.; Wu, P.; Marion-Tsukamaki, R.; Mackey, Z.B.; Brinen, L.S.
    Crystal Structures of TbCatB and rhodesain, potential chemotherapeutic targets and major cysteine proteases of Trypanosoma brucei (2010), PLoS Negl. Trop. Dis., 4, e701.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.B6

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged version expressed in Pichia pastoris Trypanosoma brucei

Crystallization (Commentary)

Crystallization (Comment) Organism
TbCatB/CA074 complex, hanging-drop method Trypanosoma brucei

Protein Variants

Protein Variants Comment Organism
N216D predicted glycosylation site Trypanosoma brucei

Inhibitors

Inhibitors Comment Organism Structure
N-(L-3-trans-propylcarbamoyl-oxirane-2-carbonyl)-L-Ile-L-Pro CA074, irreversible inhibitor Trypanosoma brucei

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from supernatant, immobilized metal ion affinity chromatography (Ni2+), ion-exchange chromatography (Mono-Q) Trypanosoma brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Z-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Trypanosoma brucei Z-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?

Synonyms

Synonyms Comment Organism
TbCatB cathepsin B-like enzyme Trypanosoma brucei