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Literature summary for 3.4.22.B5 extracted from

  • Lanfranco, M.F.; Loayza-Muro, R.; Clark, D.; Nunez, R.; Zavaleta, A.I.; Jimenez, M.; Meldal, M.; Coombs, G.H.; Mottram, J.C.; Izidoro, M.; Juliano, M.A.; Juliano, L.; Arevalo, J.
    Expression and substrate specificity of a recombinant cysteine proteinase B of Leishmania braziliensis (2008), Mol. Biochem. Parasitol., 161, 91-100.
    View publication on PubMed
Search for more literature for 3.4.22.B5

Cloned(Commentary)

Cloned (Comment) Organism
expression of CPB2.3DELTACTE (truncated CPB2.3 lacking the C-terminal extension) in Escherichia coli Leishmania braziliensis

Inhibitors

Inhibitors Comment Organism Structure
2-aminobenzoyl-KLRGSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
 Leishmania braziliensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0006
-
 2-aminobenzoyl-FRAK(Dnp)-NH2 pH 6.5, 37°C Leishmania braziliensis
0.0024
-
 2-aminobenzoyl-FRAK(Dnp)-OH pH 6.5, 37°C Leishmania braziliensis
0.015
-
 2-aminobenzoyl-KLRSFKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
0.018
-
 2-aminobenzoyl-KLHSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
0.021
-
 2-aminobenzoyl-FLRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
 Leishmania braziliensis
0.024
-
 2-aminobenzoyl-KFRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
0.029
-
 2-aminobenzoyl-NLRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
 Leishmania braziliensis
0.029
-
 2-aminobenzoyl-KLRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
0.029
-
 2-aminobenzoyl-KLRSPKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
0.035
-
 2-aminobenzoyl-KLRNSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
0.038
-
 2-aminobenzoyl-KLRGSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
0.038
-
 2-aminobenzoyl-KLLSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
0.041
-
 2-aminobenzoyl-KGRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
0.043
-
 2-aminobenzoyl-KLRFSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
0.044
-
 2-aminobenzoyl-KLCSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
0.052
-
 2-aminobenzoyl-KLNSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
0.052
-
 2-aminobenzoyl-KLRSGKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
0.058
-
 2-aminobenzoyl-ELRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
 Leishmania braziliensis
0.059
-
 2-aminobenzoyl-KLRSEKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
0.078
-
 2-aminobenzoyl-KLRSLKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
0.084
-
 2-aminobenzoyl-KLFSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
0.085
-
 2-aminobenzoyl-KLESSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
0.103
-
 2-aminobenzoyl-KLRRSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
0.106
-
 2-aminobenzoyl-KLRESKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
0.124
-
 2-aminobenzoyl-KRRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
0.172
-
 2-aminobenzoyl-LLRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
 Leishmania braziliensis
0.351
-
 2-aminobenzoyl-KLRSRKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
0.406
-
 2-aminobenzoyl-KNRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
0.435
-
 2-aminobenzoyl-KERSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis

Organism

Organism UniProt Comment Textmining
Leishmania braziliensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant CPB2.3DELTACTE (CPB2.3 lacking the C-terminal extension) Leishmania braziliensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminobenzoyl-ELRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
-
 Leishmania braziliensis 2-aminobenzoyl-ELR + SSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
 ?
2-aminobenzoyl-FLRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
-
 Leishmania braziliensis 2-aminobenzoyl-FLR + SSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
 ?
2-aminobenzoyl-FRAK(Dnp)-NH2 + H2O
-
 Leishmania braziliensis ?
-
 ?
2-aminobenzoyl-FRAK(Dnp)-OH + H2O
-
 Leishmania braziliensis ?
-
 ?
2-aminobenzoyl-KERSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
-
 Leishmania braziliensis 2-aminobenzoyl-KER + SSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
 ?
2-aminobenzoyl-KFRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
-
 Leishmania braziliensis 2-aminobenzoyl-KFR + SSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
 ?
2-aminobenzoyl-KGRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
-
 Leishmania braziliensis 2-aminobenzoyl-KGR + SSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
 ?
2-aminobenzoyl-KLCSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
-
 Leishmania braziliensis 2-aminobenzoyl-KLC + SSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
 ?
2-aminobenzoyl-KLESSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
-
 Leishmania braziliensis 2-aminobenzoyl-KLE + SSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
 ?
2-aminobenzoyl-KLFSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
-
 Leishmania braziliensis 2-aminobenzoyl-KLF + SSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
 ?
2-aminobenzoyl-KLHSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
-
 Leishmania braziliensis 2-aminobenzoyl-KLH + SSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
 ?
2-aminobenzoyl-KLLSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
-
 Leishmania braziliensis 2-aminobenzoyl-KLL + SSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
 ?
2-aminobenzoyl-KLNSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
-
 Leishmania braziliensis 2-aminobenzoyl-KLN + SSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
 ?
2-aminobenzoyl-KLRESKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
-
 Leishmania braziliensis 2-aminobenzoyl-KLR + ESKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
 ?
2-aminobenzoyl-KLRFSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
-
 Leishmania braziliensis 2-aminobenzoyl-KLR + FSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
 ?
2-aminobenzoyl-KLRGSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
-
 Leishmania braziliensis 2-aminobenzoyl-KLR + GSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
 ?
2-aminobenzoyl-KLRNSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
-
 Leishmania braziliensis 2-aminobenzoyl-KLR + NSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
 ?
2-aminobenzoyl-KLRRSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
-
 Leishmania braziliensis 2-aminobenzoyl-KLR + RSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
 ?
2-aminobenzoyl-KLRSEKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
-
 Leishmania braziliensis 2-aminobenzoyl-KLR + SEKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
 ?
2-aminobenzoyl-KLRSFKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
-
 Leishmania braziliensis 2-aminobenzoyl-KLR + SFKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
 ?
2-aminobenzoyl-KLRSGKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
-
 Leishmania braziliensis 2-aminobenzoyl-KLR + SGKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
 ?
2-aminobenzoyl-KLRSLKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
-
 Leishmania braziliensis 2-aminobenzoyl-KLR + SLKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
 ?
2-aminobenzoyl-KLRSPKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
-
 Leishmania braziliensis 2-aminobenzoyl-KLR + SPKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
 ?
2-aminobenzoyl-KLRSRKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
-
 Leishmania braziliensis 2-aminobenzoyl-KLR + SRKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
 ?
2-aminobenzoyl-KLRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
-
 Leishmania braziliensis 2-aminobenzoyl-KLR + SSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
 ?
2-aminobenzoyl-KNRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
-
 Leishmania braziliensis 2-aminobenzoyl-KNR + SSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
 ?
2-aminobenzoyl-KRRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
-
 Leishmania braziliensis 2-aminobenzoyl-KRR + SSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
 ?
2-aminobenzoyl-LLRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
-
 Leishmania braziliensis 2-aminobenzoyl-LLR + SSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
 ?
2-aminobenzoyl-NLRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
-
 Leishmania braziliensis 2-aminobenzoyl-NLR + SSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
 ?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Leishmania braziliensis ?
-
 ?
additional information peptides containing the basic amino acids Arg and His at position P1 are successfully hydrolyzed, although other substitutions with acidic (Glu), hydrophobic (Phe, Leu) and non-charged (Asn, Cys) residues are also well accepted by rCPB2.3DELTACTE. Peptides with Leu and Phe at P2 position are superior substrates. High preference for peptides with Phe and Ser at position P1' position, followed by those with Gly and Leu, and to a lesser extent by Arg and Glu. The carboxydipeptidase activity of rCPB2.3DELTACTE, although more than one order of magnitude lower than its endopeptidase activity, may play a role in peptide or protein processing during the life cycle of Leishmania braziliensis Leishmania braziliensis ?
-
 ?

Synonyms

Synonyms Comment Organism
CPB2.3 cysteine proteinase derived from the 2.3 kb DNA fragment Leishmania braziliensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.92
-
 2-aminobenzoyl-FRAK(Dnp)-OH pH 6.5, 37°C Leishmania braziliensis
4.05
-
 2-aminobenzoyl-FRAK(Dnp)-NH2 pH 6.5, 37°C Leishmania braziliensis
10
-
 2-aminobenzoyl-KLRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
130
-
 2-aminobenzoyl-KLLSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
255
-
 2-aminobenzoyl-KLHSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
276
-
 2-aminobenzoyl-KLRSGKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
286
-
 2-aminobenzoyl-KLRNSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
296
-
 2-aminobenzoyl-LLRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
 Leishmania braziliensis
302
-
 2-aminobenzoyl-KLRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
322
-
 2-aminobenzoyl-KLRGSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
334
-
 2-aminobenzoyl-KLRSLKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
339
-
 2-aminobenzoyl-KLRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
360
-
 2-aminobenzoyl-KLCSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
372
-
 2-aminobenzoyl-KLESSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
375
-
 2-aminobenzoyl-KLRRSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
404
-
 2-aminobenzoyl-KLRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
424
-
 2-aminobenzoyl-FLRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
 Leishmania braziliensis
491
-
 2-aminobenzoyl-KLNSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
496
-
 2-aminobenzoyl-ELRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
 Leishmania braziliensis
543
-
 2-aminobenzoyl-KLRFSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
552
-
 2-aminobenzoyl-KLRSRKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
567
-
 2-aminobenzoyl-KLRSPKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
572
-
 2-aminobenzoyl-KLFSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
583
-
 2-aminobenzoyl-NLRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
 Leishmania braziliensis
614
-
 2-aminobenzoyl-KLRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
614
-
 2-aminobenzoyl-KLRSEKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
631
-
 2-aminobenzoyl-KLRESKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
912
-
 2-aminobenzoyl-KLRSFKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
1026
-
 2-aminobenzoyl-KLRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 substrate: benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin Leishmania braziliensis

pH Range

pH Minimum pH Maximum Comment Organism
4.5 7.5 pH 4.5: about 70% of maximal activity, pH 7.5: about 45% of maximal activity, substrate: benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin Leishmania braziliensis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000016
-
 2-aminobenzoyl-KLPSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis
0.1
-
 2-aminobenzoyl-KLRGSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) pH 6.5, 37°C Leishmania braziliensis