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Literature summary for 3.4.22.B5 extracted from

  • Juliano, M.A.; Brooks, D.R.; Selzer, P.M.; Pandolfo, H.L.; Judice, W.A.; Juliano, L.; Meldal, M.; Sanderson, S.J.; Mottram, J.C.; Coombs, G.H.
    Differences in substrate specificities between cysteine protease CPB isoforms of Leishmania mexicana are mediated by a few amino acid changes (2004), Eur. J. Biochem., 271, 3704-3714.
    View publication on PubMed
Search for more literature for 3.4.22.B5

Application

Application Comment Organism
medicine large preference for Pro in the P2' position for the hydrolytic activity of CPB3, which may be relevant to a role in the activation mechanism of th Leishmania mexicana Leishmania mexicana

Cloned(Commentary)

Cloned (Comment) Organism
in Escherichia coli Leishmania mexicana

Protein Variants

Protein Variants Comment Organism
H84Y specificity of S3 to S3' subsites is influenced greatly by the modifications at amino acids 60, 61, 64 and 84 Leishmania mexicana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
 Abz-FR(2,4-dinitrophenyl)-epsilon-NH2-Lys rCPB3 Leishmania mexicana
0.85
-
 Abz-FRA(2,4-dinitrophenyl)-epsilon-NH2-Lys-amide rH84Y Leishmania mexicana
1.2
-
 Abz-FRA(2,4-dinitrophenyl)-epsilon-NH2-Lys-amide rCPB3 Leishmania mexicana
1.9
-
 Abz-FR(2,4-dinitrophenyl)-epsilon-NH2-Lys rH84Y Leishmania mexicana
2.2
-
 Abz-FRA(2,4-dinitrophenyl)-epsilon-NH2-Lys rH84Y Leishmania mexicana
2.9
-
 Abz-FRA(2,4-dinitrophenyl)-epsilon-NH2-Lys rCPB3 Leishmania mexicana
3.3
-
 Abz-FRF(2,4-dinitrophenyl)-epsilon-NH2-Lys rCPB3 Leishmania mexicana
3.9
-
 Abz-FRF(2,4-dinitrophenyl)-epsilon-NH2-Lys rH84Y Leishmania mexicana
4.7
-
 Abz-ARF(2,4-dinitrophenyl)-epsilon-NH2-Lys rH84Y Leishmania mexicana

Organism

Organism UniProt Comment Textmining
Leishmania mexicana
-
-
-
Leishmania mexicana MNYC/BZ/M379
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
promastigote
-
 Leishmania mexicana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Abz-ARF(2,4-dinitrophenyl)-epsilon-NH2-Lys + H2O
-
 Leishmania mexicana ?
-
 ?
Abz-ARF(2,4-dinitrophenyl)-epsilon-NH2-Lys + H2O
-
 Leishmania mexicana MNYC/BZ/M379 ?
-
 ?
Abz-FR(2,4-dinitrophenyl)-epsilon-NH2-Lys + H2O
-
 Leishmania mexicana ?
-
 ?
Abz-FR(2,4-dinitrophenyl)-epsilon-NH2-Lys + H2O
-
 Leishmania mexicana MNYC/BZ/M379 ?
-
 ?
Abz-FRA(2,4-dinitrophenyl)-epsilon-NH2-Lys + H2O
-
 Leishmania mexicana ?
-
 ?
Abz-FRA(2,4-dinitrophenyl)-epsilon-NH2-Lys + H2O
-
 Leishmania mexicana MNYC/BZ/M379 ?
-
 ?
Abz-FRA(2,4-dinitrophenyl)-epsilon-NH2-Lys-amide + H2O
-
 Leishmania mexicana ?
-
 ?
Abz-FRF(2,4-dinitrophenyl)-epsilon-NH2-Lys + H2O
-
 Leishmania mexicana ?
-
 ?
Abz-peptidyl-Q-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Leishmania mexicana ?
-
 ?
Abz-RRF(2,4-dinitrophenyl)-epsilon-NH2-Lys + H2O
-
 Leishmania mexicana ?
-
 ?
Gelatin + H2O
-
 Leishmania mexicana ?
-
 ?
Gelatin + H2O
-
 Leishmania mexicana MNYC/BZ/M379 ?
-
 ?
additional information hydrolysis of peptides derived from Abz-KLRFSKQ-EDDnp, containing modifications at positions P1, P2 and P3, P1 to P3 Leishmania mexicana ?
-
 ?
additional information hydrolysis of peptides derived from Abz-KLRFSKQ-EDDnp, containing modifications at positions P1, P2 and P3, P1 to P3 Leishmania mexicana MNYC/BZ/M379 ?
-
 ?

Synonyms

Synonyms Comment Organism
CPB18
-
 Leishmania mexicana
CPB2.8
-
 Leishmania mexicana
CPB3
-
 Leishmania mexicana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.004
-
 Abz-ARF(2,4-dinitrophenyl)-epsilon-NH2-Lys rH84Y Leishmania mexicana
0.14
-
 Abz-FR(2,4-dinitrophenyl)-epsilon-NH2-Lys rCPB3 Leishmania mexicana
0.23
-
 Abz-FRF(2,4-dinitrophenyl)-epsilon-NH2-Lys rCPB3 Leishmania mexicana
0.24
-
 Abz-FR(2,4-dinitrophenyl)-epsilon-NH2-Lys rH84Y Leishmania mexicana
0.35
-
 Abz-FRA(2,4-dinitrophenyl)-epsilon-NH2-Lys rCPB3 Leishmania mexicana
0.46
-
 Abz-FRA(2,4-dinitrophenyl)-epsilon-NH2-Lys rH84Y Leishmania mexicana
0.51
-
 Abz-FRF(2,4-dinitrophenyl)-epsilon-NH2-Lys rH84Y Leishmania mexicana
0.9
-
 Abz-FRA(2,4-dinitrophenyl)-epsilon-NH2-Lys-amide rCPB3 Leishmania mexicana
1.14
-
 Abz-FRA(2,4-dinitrophenyl)-epsilon-NH2-Lys-amide rH84Y Leishmania mexicana