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Literature summary for 3.4.22.B5 extracted from

  • Alves, L.C.; Melo, R.L.; Cezari, M.H.S.; Sanderson, S.J.; Mottram, J.C.; Coombs, G.H.; Juliano, L.; Juliano, M.A.
    Analysis of the S2 subsite specificities of the recombinant cysteine proteinases CPB of Leishmania mexicana, and cruzain of Trypanosoma cruzi, using fluorescent substrates containing non-natural basic amino acids (2001), Mol. Biochem. Parasitol., 117, 137-143.
    View publication on PubMed
Search for more literature for 3.4.22.B5

Inhibitors

Inhibitors Comment Organism Structure
benzoyl-(4-aminomethyl-cyclohexyl-alanine)-R-4-methylcoumarin-7-amide
-
 Leishmania mexicana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0014
-
 benzoyl-FR-4-methylcoumarin-7-amide pH 6.3, 37°C Leishmania mexicana
0.0038
-
 benzoyl-(4-aminomethyl-phenylalanine)-R-4-methylcoumarin-7-amide pH 6.3, 37°C Leishmania mexicana
0.0114
-
 benzoyl-(3-pyridylalanine)-R-4-methylcoumarin-7-amide pH 6.3, 37°C Leishmania mexicana
0.012
-
 benzoyl-RR-4-methylcoumarin-7-amide pH 6.3, 37°C Leishmania mexicana
0.021
-
 benzoyl-(4-aminomethyl-N-isopropyl-phenylalanine)-R-4-methylcoumarin-7-amide pH 6.3, 37°C Leishmania mexicana
0.035
-
 benzoyl-(4-guanidine-phenylalanine)-R-4-methylcoumarin-7-amide pH 6.3, 37°C Leishmania mexicana
0.051
-
 benzoyl-HR-4-methylcoumarin-7-amide pH 6.3, 37°C Leishmania mexicana

Organism

Organism UniProt Comment Textmining
Leishmania mexicana
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoyl-(3-pyridylalanine)-R-4-methylcoumarin-7-amide + H2O
-
 Leishmania mexicana benzoyl-(3-pyridylalanine)-R + 7-amino-4-methylcoumarin
-
 ?
benzoyl-(4-aminomethyl-N-isopropyl-phenylalanine)-R-4-methylcoumarin-7-amide + H2O
-
 Leishmania mexicana benzoyl-(4-aminomethyl-N-isopropyl-phenylalanine) + 7-amino-4-methylcoumarin
-
 ?
benzoyl-(4-aminomethyl-phenylalanine)-R-4-methylcoumarin-7-amide + H2O
-
 Leishmania mexicana benzoyl-(4-aminomethyl-phenylalanine)-R + 7-amino-4-methylcoumarin
-
 ?
benzoyl-(4-guanidine-phenylalanine)-R-4-methylcoumarin-7-amide + H2O
-
 Leishmania mexicana benzoyl-(4-guanidine-phenylalanine)-R + 7-amino-4-methylcoumarin
-
 ?
benzoyl-FR-4-methylcoumarin-7-amide + H2O
-
 Leishmania mexicana benzoyl-FR + 7-amino-4-methylcoumarin
-
 ?
benzoyl-HR-4-methylcoumarin-7-amide + H2O
-
 Leishmania mexicana benzoyl-HR + 7-amino-4-methylcoumarin
-
 ?
benzoyl-RR-4-methylcoumarin-7-amide + H2O
-
 Leishmania mexicana benzoyl-RR + 7-amino-4-methylcoumarin
-
 ?
additional information no hydrolysis of benzoyl-(4-piperidinylalanine)-R-4-methylcoumarin-7-amide and benzoyl-(4-aminocyclohexylalanine)-R-4-methylcoumarin-7-amide Leishmania mexicana ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.18
-
 benzoyl-(4-aminomethyl-phenylalanine)-R-4-methylcoumarin-7-amide pH 6.3, 37°C Leishmania mexicana
0.2
-
 benzoyl-RR-4-methylcoumarin-7-amide pH 6.3, 37°C Leishmania mexicana
0.3
-
 benzoyl-HR-4-methylcoumarin-7-amide pH 6.3, 37°C Leishmania mexicana
1.1
-
 benzoyl-(4-guanidine-phenylalanine)-R-4-methylcoumarin-7-amide pH 6.3, 37°C Leishmania mexicana
1.4
-
 benzoyl-(4-aminomethyl-N-isopropyl-phenylalanine)-R-4-methylcoumarin-7-amide pH 6.3, 37°C Leishmania mexicana
1.45
-
 benzoyl-(3-pyridylalanine)-R-4-methylcoumarin-7-amide pH 6.3, 37°C Leishmania mexicana
3.9
-
 benzoyl-FR-4-methylcoumarin-7-amide pH 6.3, 37°C Leishmania mexicana