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Literature summary for 3.4.22.B49 extracted from

  • Hernandez-Guzman, K.; Sahagun-Ruiz, A.; Vallecillo, A.J.; Cruz-Mendoza, I.; Quiroz-Romero, H.
    Construction and evaluation of a chimeric protein made from Fasciola hepatica leucine aminopeptidase and cathepsin L1 (2016), J. Helminthol., 90, 7-13.
    View publication on PubMed
Search for more literature for 3.4.22.B49

Application

Application Comment Organism
medicine a leucine aminopeptidase-cathepsin L1 chimeric protein may be used as a diagnostic tool for detection of antibodies against Fasciola hepatica in bovine sera and as an immunogen to induce protection against bovine fasciolosis Fasciola hepatica

Cloned(Commentary)

Cloned (Comment) Organism
a 25 kDa leucine aminopeptidase-cathepsin L1 chimeric protein is expressed from the pET15b plasmid in the Escherichia coli strain Rosetta (DE3) Fasciola hepatica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Collagen type I + H2O Fasciola hepatica
-
 ?
-
 ?
Collagen type III + H2O Fasciola hepatica
-
 ?
-
 ?
collagen type IV + H2O Fasciola hepatica
-
 ?
-
 ?
Fibronectin + H2O Fasciola hepatica
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Fasciola hepatica Q9GRW5
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA affinity column chromatography Fasciola hepatica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Collagen type I + H2O
-
 Fasciola hepatica ?
-
 ?
Collagen type III + H2O
-
 Fasciola hepatica ?
-
 ?
collagen type IV + H2O
-
 Fasciola hepatica ?
-
 ?
Fibronectin + H2O
-
 Fasciola hepatica ?
-
 ?

Synonyms

Synonyms Comment Organism
CL1
-
 Fasciola hepatica