Literature summary for 3.4.22.B49 extracted from

Collins, P.R.; Stack, C.M.; ONeill, S.M.; Doyle, S.; Ryan, T.; Brennan, G.P.; Mousley, A.; Stewart, M.; Maule, A.G.; Dalton, J.P.; Donnelly, S.
Cathepsin L1, the major protease involved in liver fluke (Fasciola hepatica) virulence: propetide cleavage sites and autoactivation of the zymogen secreted from gastrodermal cells (2004), J. Biol. Chem., 279, 17038-17046.

Search for more literature for 3.4.22.B49

Cloned(Commentary)

Cloned (Comment)	Organism
expression of recombinant proenzyme in Pichia pastoris	Fasciola hepatica

Inhibitors

Inhibitors	Comment	Organism	Structure
benzyloxycarbonyl-L-phenylalanyl-L-alanine-diazomethylketone(-CHN2)	-	Fasciola hepatica

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
additional information	epithelial cells, inactive proform procathepsin L1 is packaged in secretory vesicles lining the parasite gut, activation of the proform takes place following secretion of the protease into the gut lumen	Fasciola hepatica	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
24500	-	sequence analysis	Fasciola hepatica
37000	-	proenzyme, SDS-PAGE	Fasciola hepatica

Organism

Organism	UniProt	Comment	Textmining
Fasciola hepatica	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
gel filtration, proenzyme purified by affinity Ni-NTA-agarose chromatography	Fasciola hepatica

Source Tissue

Source Tissue	Comment	Organism	Textmining
gastrodermis	-	Fasciola hepatica	-
gut	-	Fasciola hepatica	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
benzyloxycarbonyl-L-phenylalanyl-L-arginine 4-methylcoumarinyl-7-amide + H2O	-	Fasciola hepatica	?	-	?

Synonyms

Synonyms	Comment	Organism
cathepsin L1	-	Fasciola hepatica
cathepsin L1 cysteine protease	-	Fasciola hepatica

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	8	-	Fasciola hepatica

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Release 2023.1 (January 2023)